fa بررسی تاثیر عصاره جلبک Sargassum tenerrimum بر بیان ژن اینترلوکین-12 در سلول های C121 لوسمی لنفوبلاستیک تخصصي Special پژوهشي Research <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">مقدمه: </span></span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">سرطان خون یا لوسمی به</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">عنوان پنجمین سرطان شایع در جهان شناخته می</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">شود. در کنار داروهای شیمیایی موجود، توجه به منابع طبیعی و ترکیبات موجود در آن&shy; ها جهت درمان انواع سرطان جلب شده است. یکی از این منابع، جلبک &shy;های دریایی هستند که سرشار از ترکیبات فعال زیستی دارای عملکردهای مختلف می&shy; باشند</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">.</span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"> بر همین اساس هدف از تحقیق حاضر بررسی اثر عصاره&shy; جلبک </span></span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Sargassum tenerrimum</span></span></i><i> </i><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">در بیان ژن اینترلوکین</span></span><span lang="FA" style="font-size:8.0pt"><span b="" baran="" style="font-family:">-</span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">12 در سلول &shy;های رده </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">C121</span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"> بود.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">مواد و روش &shy;ها:</span></span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">برای این منظور پس از نمونه &shy;برداری جلبک &shy;ها در فصل بهار از منطقه جذر و مدی سواحل بوشهر، انتقال آن &shy;ها به آزمایشگاه و شناسایی مورفولوژیک جلبک، عصاره&shy; گیری با استفاده از حلال&shy; های متانول، کلروفرم و هیدروالکل انجام شد. سلول &shy;های </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">C121</span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"> در محیط کشت </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">RPMI 1640</span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"> کشت داده شده و با غلظت &shy;های 250، 500 و 750 میکروگرم بر میلی &shy;لیتر عصاره سارگاسوم تنریموم به</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">مدت 24 ساعت تیمار شدند. پس از گذشت این زمان، </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">RNA</span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"> سلول &shy;ها استخراج شده و بیان ژن اینترلوکین 12 </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">(IL-12)</span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"> با استفاده از </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Real Time PCR</span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"> ارزیابی شد. </span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">نتایج</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">:</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">نتایج حاصل نشان داد که بیان ژن </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">IL-12</span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"> در رده سلولی </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">C121</span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"> تیمار شده با تمامی غلظت‌های مورد مطالعه (250، 500 و 750 میکروگرم بر</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">میلی‌لیتر) عصاره‌های کلروفرمی، هیدروالکلی و متانولی </span></span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Sargassum tenerrimum</span></span></i><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"> نسبت به گروه کنترل افزایش می &shy;یابد. بیش</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">ترین اثر را در افزایش بیان این ژن، غلظت &shy;های 500 میکروگرم بر میلی &shy;لیتر عصاره کلروفرمی و متانولی داشتند (0/05</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">p<</span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">). یافته</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">ها نشان می</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">دهد که نوع و غلظت عصاره می تواند تأثیر قابل توجهی بر پاسخ ایمنی با واسطه </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">IL-12</span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"> داشته باشد.</span></span></span></span></span></span></span></span><br> <span class="MsoPlaceholderText" style="color:gray"><b><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:"><span style="color:black">بحث و نتیجه&shy; گیری: </span></span></span></span></b></span><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:">با توجه به افزایش بیان </span></span></span><span style="font-size:10.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">IL-12</span></span></span><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:"> تحت اثر</span></span></span> <span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:">عصاره&shy; سارگاسوم تنریموم می &shy;توان نتیجه</span></span></span> <span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:">گرفت</span></span></span> <span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:">که عصاره این</span></span></span> <span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:">جلبک دارای پتانسیل ضدسرطانی علیه لوسمی لنفوبلاستیک است که تحقیقات بیش</span></span></span> <span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:">تر در جهت یافتن ماده موثره موجود در عصاره و هم</span></span></span> <span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:">چنین شناخت مکانیسم &shy;های دخیل در آن می &shy;تواند به بالفعل کردن پتانسیل بالقوه ضدسرطانی آن کمک نماید.</span></span></span></div> <div style="text-align: justify;"><span style="font-size:12pt"><span style="font-family:&quot;Times New Roman&quot;,serif"><b>Introduction:</b> <span style="font-size:11.0pt">Leukemia is the fifth most common cancer in the world. In addition to existing chemotherapy drugs, attention has been drawn to natural resources and their constituents for the treatment of various cancers. One of these resources is marine algae, which are rich in biologically active compounds with various functions. Therefore, the aim of the present study was to investigate the effect of <i>Sargassum</i> <i>tenerrimum</i> extract on the expression of the interleukin-12 (IL-12) gene in C121 cell line.</span></span></span><br> <span style="font-size:12pt"><span style="font-family:&quot;Times New Roman&quot;,serif"><b>Materials & Methods:</b> <span style="font-size:11.0pt">For this purpose, after sampling the algae in the spring from the intertidal zone of the coasts of Bushehr, transferring them to the laboratory and morphological identification of the algae, extraction was performed using methanol, chloroform and ethanol solvents. C121 cells were cultured in RPMI 1640 culture medium and treated with concentrations of 250, 500 and 750 micrograms per milliliter of <i>Sargassum</i> <i>tenerrimum</i> extract for 24 hours. After this time, the RNA of the cells was extracted and the expression of the interleukin-12 (IL-12) gene was evaluated using the Real-Time PCR method.</span></span></span><br> <span style="font-size:12pt"><span style="font-family:&quot;Times New Roman&quot;,serif"><b>Results:</b> <span style="font-size:11.0pt">The results showed that the expression of the IL-12 gene in the C121 cell line treated with all the studied concentrations (250, 500 and 750 micrograms per milliliter) of chloroform, hydroalcoholic and methanolic extracts of <i>Sargassum tenerrimum</i> was increased compared to the control group. The greatest effect on increasing the expression of this gene was observed in the concentrations of 500 micrograms per milliliter of chloroform and methanolic extract (p<0.05). The findings suggest that the type and concentration of the extract can have a significant effect on the IL-12-mediated immune response.</span></span></span><br> <b><span style="font-size:11.0pt"><span style="line-height:107%"><span style="font-family:&quot;Calibri&quot;,sans-serif">Conclusion:</span></span></span></b> <span style="font-size:11.0pt"><span style="line-height:107%"><span style="font-family:&quot;Calibri&quot;,sans-serif">Based on the increase in IL-12 expression under the effect of <i>Sargassum</i> <i>tenerrimum</i> extract, it can be concluded that the extract of this alga has anti-cancer potential against lymphoblastic leukemia. Further research to find the active ingredient present in the extract and also to understand the mechanisms involved in it can help to realize its potential anti-cancer potential.</span></span></span></div> جلبک دریایی, ضد سرطان, خلیج فارس, عصاره دریایی, Real-Time PCR Marine algae, Anti-cancer, Persian Gulf, Marine extract, Real-Time PCR 51 58 http://journalaer.com/browse.php?a_code=A-10-2-61&slc_lang=fa&sid=1 Maryam Nourniaei مریم نورنیایی 10031947532846001105 10031947532846001105 No Hoda Khaledi هدا خالدی hodakhaledi@inio.ac.ir 10031947532846001106 10031947532846001106 Yes Ali Khodadadi علی خدادادی 10031947532846001107 10031947532846001107 No Seyedeh Maryam Mousavi سیده مریم موسوی 10031947532846001108 10031947532846001108 No Rezvan Attari رضوان عطاری 10031947532846001109 10031947532846001109 No