fa بررسی اثر عصاره زردچوبه (Curcuma longa) و سیر (Allium sativum) بر برخی از فاکتورهای خونی موش نر نژاد ویستار تخصصي Special پژوهشي Research <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">مقدمه:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">هدف از مطالعه حاضر بررسی اثرات ترکیبی عصاره سیر و زردچوبه در برابر آسیب ناشی از آفلاتوکسین </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">B1</span></span><span style="font-size:12.0pt"><span b="" baran="" style="font-family:"> بود که بر روی 64 سر موش صحرایی نر 12-10 هفته، با میانگین</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"> وزنی 300-250 گرم در قالب یک طرح کاملاً تصادفی به‌صورت آزمایش فاکتوریل (2&times;2&times;2) در هشت گروه و به</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">‌مدت 28 روز انجام گردید. </span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">مواد و روش ها:</span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"> در این مطالعه، سه عامل مورد بررسی قرار گرفت که هر&lrm;کدام دو سطح داشتند. شامل دو سطح آفلاتوکسین </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">B1</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"> (صفر و 100 میکروگرم در کیلوگرم وزن بدن)، 2 سطح زردچوبه (صفر و 100 میلی&rlm; گرم در کیلوگرم وزن بدن) و 2 سطح سیر (صفر و 50 میلی&rlm; گرم در کیلوگرم وزن بدن) برای هر تیمار 8 تکرار در نظر گرفته شد. در تمام گروه‌ها، آفلاتوکسین و عصاره‌‌ها به‌صورت خوراکی داده شد. در روز 28 دوره آزمایش، موش &rlm;های</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">صحرایی</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">با</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">کتامین&nbsp;</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">بی</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">هوش شدند</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">و پس</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">از</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">بازکردن</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">حفره</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">صدری،</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">خونگیری</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">از</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">قلب انجام شد. آنالیز</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">نمونه&rlm; های پلاسما به</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">منظور تعیین میزان اسید ‌اوریک، کراتینین، پروتئین، آلبومین، گلوکز، تری&rlm; گلیسرید، کلسترول،گاما گلوتامیل ترانسفراز، لاکتات دهیدروژناز، لیپوپروتئین با چگالی پایین </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">)</span></span> <span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">(LDL</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"> و لیپوپروتئین با چگالی بالا (</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">HDL</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">) صورت گرفت. </span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">نتابج: </span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">نتایج نشان داد که آفلاتوکسین </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">B1</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"> فعالیت لاکتات دهیدروژناز، گاماگلوتامیل </span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">ترانسفراز، کراتینین و </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">LDL</span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"> سرم خون را به &rlm;طور معنی&rlm; داری افزایش می &rlm;دهد. </span></span></span></span></span></span></span><br> <b><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:">بحث و نتیجه</span></span></span> <span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:">گیری:</span></span></span></b><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:"> درمان</span></span></span> <span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:">موش صحرایی</span></span></span> <span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:">آلوده با عصاره زردچوبه و عصاره</span></span></span> <span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:">سیر باعث</span></span></span> <span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:">کاهش معنی &rlm;دار لاکتات دهیدروژناز، گاماگلوتامیل ترانسفراز و&nbsp;</span></span></span><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">LDL</span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">&nbsp;د</span></span></span></span></span></span></span><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:">ر مقایسه با تیمار آفلاتوکسین </span></span></span><span style="font-size:10.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">B1</span></span></span><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:">&nbsp; &nbsp;شد. عصاره سیر توانست میزان </span></span></span><span style="font-size:10.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">LDL</span></span></span><span dir="RTL" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:"> پلاسما موش &rlm;های صحرایی را به &rlm;طور معنی &rlm;داری کاهش دهد.&nbsp;</span></span></span><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:">به‌طورکلی یافته &rlm;های تحقیق حاضر نشان می &rlm;دهد&nbsp;استفاده از ترکیب&nbsp;زردچوبه و سیر به</span></span></span> <span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:">صورت خوراکی عوارض زیان&rlm; بار آفلاتوکسین </span></span></span><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:">را کاهش می&rlm; دهد.</span></span></span></div> <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-family:&quot;Times New Roman&quot;,serif"><span style="color:black">Introduction:</span></span></b> <span style="font-family:&quot;Times New Roman&quot;,serif">Because aflatoxin is very resistant to heat and this toxin easily enters the human body through the food chain and consumption of dairy and livestock products and causes poisoning, it is necessary to seek solutions and materials to reduce the side effects of aflatoxins. This study investigates the combined effects of turmeric and garlic extract for this purpose for the first time in this context. It is hoped that new results can be achieved to improve and reduce the risks of toxins, given the antioxidant role of these two substances. The aim of the present study is to investigate the combined effects of turmeric and garlic extract in improving the harm caused by aflatoxin B1.</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-family:&quot;Times New Roman&quot;,serif">Materials & Methods:</span></b><span style="font-family:&quot;Times New Roman&quot;,serif"> In this experimental study, 3 factors were investigated, each with 2 levels. Including two levels of aflatoxin B1 (0 and 100 micrograms per kilogram of body weight), 2 levels of turmeric (0 and 100 mg per kilogram of body weight), and 2 levels of garlic (0 and 50 mg per kilogram of body weight). Therefore, the total number of treatments was 8, and 8 replicates were considered for each treatment, and the total number of experimental units was 64 rats. On day 28 of the experimental period, rats were anesthetized with ketamine, and after opening the thoracic cavity, blood was collected from the heart. Plasma samples were analyzed to determine the levels of uric acid, creatinine, protein, albumin, glucose, triglycerides, cholesterol, GGT, LDH, LDL, and HDL.</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-family:&quot;Times New Roman&quot;,serif">Results:</span></b><span style="font-family:&quot;Times New Roman&quot;,serif"> The results showed that aflatoxin B1 significantly increased the activity of lactate dehydrogenase, gamma-glutamyl transferase, creatinine and LDL in serum. </span></span></span></span><br> <b><span style="font-size:11.0pt"><span style="line-height:107%"><span style="font-family:&quot;Times New Roman&quot;,serif">Conclusion:</span></span></span></b><span style="font-size:11.0pt"><span style="line-height:107%"><span style="font-family:&quot;Times New Roman&quot;,serif"> Treatment of infected rats with turmeric extract and garlic extract significantly reduced lactate dehydrogenase, gammaglutamyl transferase and LDL, compared to aflatoxin B1 treatment. Garlic extract was able to significantly reduce the serum LDL level of rats.Therefore, the findings of the present study indicate that the use of a combination of turmeric and garlic may reduces the harmful effects of aflatoxin B1.</span></span></span></div> موش صحرایی, آفلاتوکسین B1, عصاره زردچوبه, عصاره سیر, متابولیت ‌های خون Rat, Aflatoxin B1, Turmeric extract, Garlic extract, Blood metabolites 19 26 http://journalaer.com/browse.php?a_code=A-10-2-66&slc_lang=fa&sid=1 Hadi Sarir هادی سریر 10031947532846001130 10031947532846001130 No Hossein Naimipour Younesi حسین نعیمی پوریونسی 10031947532846001131 10031947532846001131 No Masood Didarkhah مسعود دیدارخواه masooddidarkhah@birjand.ac.ir 10031947532846001132 10031947532846001132 Yes