Journal of Animal Environmental Research
پژوهش های محیط زیست جانوری
Journalaer
Basic Sciences
http://journalaer.com
1
admin
3115-7904
10.22034/aer
fa
jalali
1404
5
1
gregorian
2025
8
1
1
2
online
1
fulltext
fa
تاثیر یک دوره هم افزایی تمرین شنا در آب گرم و سرد به همراه مصرف مکمل دارچین بر برخی شاخص های آپوپتوزی بافت قلب رت های دیابتی توسط استرپتوزوتوسین
Synergistic Effect of a Course of Swimming Training in Hot and Cold Water Along with the Consumption of Cinnamon Supplement on Apoptotic Indices of The Cardiac Tissue in Streptozotocin-Induced Diabetic Rats
تخصصي
Special
پژوهشي
Research
<div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">مقدمه: </span></span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">یافته ­های اخیر نشان داده است که فعالیت بدنی و استفاده از آنتی اکسیدان­ ها می ­توانند منجر به کاهش عواقب ناشی از بیماری دیابت گردند. مطالعه حاضر با هدف بررسی اثر هم ­افزایی یک دوره تمرین شنا در آب گرم و سرد و مکمل­ دهی با دارچین بر بیان شاخص ­های آپوپتوزی بافت قلب (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Bax</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black"> و</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Bcl-2</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">) در رت­ های دیابتی، صورت گرفت. </span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">مواد و روش</span></span></span></b><b><span dir="LTR" lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">‎</span></span></span></b><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">ها:</span></span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">مطالعه حاضر یک</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">تحقیق </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">بنیادی تجربی با </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">طرح پس آزمون همراه با گروه کنترل بود که روی </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">49 موش ماده نژاد اسپراگوداولی (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Sprague Dawley</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">) بالغ صورت گرفت. </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">تعداد 7 سر موش به</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">عنوان گروه کنترل سالم </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">و </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">42 سر موش دیابتی شده در گروه­ های 1) کنترل دیابتی، 2) دیابتی تمرین شنا در دمای 5 درجه سانتی</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">گراد، 3) دیابتی و مصرف دارچین، 4) دیابتی و تمرین شنا در دمای 5 درجه سانتی</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">گراد و مصرف دارچین، 5) دیابتی تمرین شنا در دمای 36 درجه سانتی</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">گراد و 6) دیابتی و تمرین شنا در دمای 36 درجه سانتی</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">گراد و مصرف</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">دارچین قرار</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">گرفتند. </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">تمرین شنا به</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">مدت 8 هفته در آب با دمای 5 و 36 درجه سانتی­ گراد صورت</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">گرفت. </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">عصاره آبی دارچین با دوز 200 میلی</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">گرم به ازای هر</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">کیلوگرم از وزن بدن</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">حیوانات به</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">صورت خوراکی تجویز شد.</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black"> اندازه ­گیری </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">بیان ژن ­های </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Bax</span></span></span><span style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black"> و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Bcl2</span></span></span><b> </b><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">با استفاده از واکنش زنجیره ایی پلیمراز (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Real time PCR</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">) صورت گرفت.</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black"></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">نتایج: </span></span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">مصرف<b> </b>دارچین، تمرین شنا و ترکیب این دو مداخله بر میزان </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">ژن­ های</span></span></span><b> </b><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">BAX</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">و</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"> BCL2 </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">بافت</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black"> قلب رت­های دیابتی شده اثر معنی ­دار</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">داشت.</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">میانگین بیان ژن</span></span></span> <span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">BAX</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">در</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">گروه دیابتی+ تمرین در دمای</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">سرد (0/03=</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">P</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">؛</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">0/259-=</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">MD</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">)،</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">گروه دیابتی+</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">تمرین در آب گرم (0/001=</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">P</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">؛ 0/875- =</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">MD</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">)، گروه دیابتی+ تمرین در دمای سرد+دارچین (0/001=</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">P</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">؛ 1/291 =</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">MD</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">) و گروه دیابتی+ تمرین در</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">دمای</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">گرم+دارچین</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">(0/001=</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">P</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">؛</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">1/673- =</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">MD</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">) کاهش معنی ­داری نسبت به</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">گروه</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">کنترل</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">دیابتی داشت. </span></span></span></span></span></span></span></span><br>
<b><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:"><span style="color:black">بحث و نتیجه ­گیری: </span></span></span></span></b><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:"><span style="color:black">به</span></span></span></span> <span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:"><span style="color:black">نظر می ­رسد که هشت ﻫﻔﺘﻪ ﺗﻤﺮﻳﻦ ﺗﻨﺎوﺑﻰ ﺷﺪﻳﺪ ﻣﻰﺗﻮاﻧﺪ با ﻛﺎﻫﺶ ﺑﻴﺎن ژن </span></span></span></span><span style="font-size:10.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black">BAX</span></span></span></span><span dir="RTL" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:"><span style="color:black"> و اﻓﺰاﻳﺶ ژن </span></span></span></span><span style="font-size:10.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="color:black">BCL-2</span></span></span></span><span dir="RTL" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:"><span style="color:black"> در ﻣﻴﻮﻛﺎرد ﻣﻮش</span></span></span></span> <span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:"><span style="color:black">ﻫﺎی ﻣﺒﺘﻼ ﺑﻪ دﻳﺎﺑﺖ، آﭘﻮﭘﺘﻮز را در ﺑﻄﻦ ﭼﭗ ﻛﺎﻫﺶ داده و اﺣﺘﻤﺎﻻً ﻛﺎردﻳﻮﻣﻴﻮﭘﺎﺗﻰ دﻳﺎﺑﺘﻰ را ﺑﻬﺒﻮد ﺑﺨﺸﺪ.</span></span></span></span></div>
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<td style="padding: 0in 12px; text-align: justify;" valign="top"><span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-family:"Times New Roman",serif">Introduction:</span></b> <span style="font-family:"Times New Roman",serif">Recent findings have demonstrated that physical activity and the use of antioxidants can mitigate the consequences of diabetes. The present study aimed to investigate the effect of swimming training in hot and cold water in combination with cinnamon supplementation on the expression of apoptotic indices of heart tissue (Bax and Bcl-2) in diabetic rats<b>.</b></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-family:"Times New Roman",serif">Materials </span></b><span dir="RTL" lang="FA" style="font-family:"Times New Roman",serif">&</span><b><span style="font-family:"Times New Roman",serif"> Methods:</span></b><span style="font-family:"Times New Roman",serif"> The present study was experimental basic research with a post-test design along with a control group, which was conducted on 49 female mice of the Spraguedauli breed. The number of 7 mice as a control group and 42 healthy mice in groups 1) control, 2) training, training at 5°C, 3°C, selection and consumption of cinnamon, 4) selection and training in the swimming temperature of 5 °C and consumption of cinnamon, 5) heat the training exercise at the temperature of 36 °C and 6 °C. The training was done for 8 weeks in water with a temperature of 5 and 36 degrees Celsius. Aqueous extract of cinnamon is prescribed at a dose of 200 mg per meal of the animal's body weight. The expression of Bax and Bcl2 genes was measured using polymerase chain reaction.</span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-family:"Times New Roman",serif">Results:</span></b><span style="font-family:"Times New Roman",serif"> Cinnamon consumption, swimming exercise, and a combination of these two interventions had a significant effect on BAX and BCL2 genes in the heart tissue of diabetic rats. The mean expression of the BAX gene in the diabetic group + exercise in cold temperature (P=0.03; MD=-0.259), diabetic group + exercise in hot water (P=0.001, MD=-0.875), group Diabetic+exercise in cold temperature+cinnamon (P=0.001; MD=1.291), and diabetic group+exercise in hot temperature+cinnamon (P=0.001; MD=1.673) significantly decreased compared to diabetic rats in control group<b>.</b></span></span></span></span><br>
<b><span style="font-size:11.0pt"><span style="line-height:107%"><span style="font-family:"Times New Roman",serif">Conclusion:</span></span></span></b><span style="font-size:11.0pt"><span style="line-height:107%"><span style="font-family:"Times New Roman",serif"> As evidenced by the obtained results, it seems that eight weeks of intense repetitive exercise could reduce the apoptosis in the left ventricle by decreasing the BAX gene expression and increasing the BCL-2 gene in the myocardium of diabetic rats and possibly improve diabetic cardiomyopathy<b>.</b></span></span></span></td>
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دارچین, دیابت, تمرین, شاخص های آپوپتوزی, موش صحرایی
Apoptotic indexs, Cinnamon, Diabetes, Exercise, Heart
87
94
http://journalaer.com/browse.php?a_code=A-10-2-75&slc_lang=fa&sid=1
Ali
Amani
علی
امانی
10031947532846001119
10031947532846001119
No
Rahmatullah
Khanmohammadi
رحمت اله
خانمحمدی
10031947532846001120
10031947532846001120
No
Seyedeh Ommolbanin
Ghasemian
سیده ام البنین
قاسمیان
ommolbanin.ghasemian@iau.ac.ir
10031947532846001121
10031947532846001121
Yes