Journal of Animal Environmental Research
پژوهش های محیط زیست جانوری
Journalaer
Basic Sciences
http://journalaer.com
1
admin
3115-7904
10.22034/aer
fa
jalali
1404
7
1
gregorian
2025
10
1
1
3
online
1
fulltext
other
بررسی اثرات کرایزین بر بیان ژن های ارکسین و هورمون تغلیظ کننده ملانین در هیپوتالاموس موش های صحرایی مدل درد القایی با فرمالین
Investigating the effects of chrysin on hypothalamic orexin and melanin-concentrating hormone gene expression in a rat model of formalin- induced pain
عمومى
General
پژوهشي
Research
<div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">مقدمه: </span></span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">کرایزین یک فلاونوئید با اثرات محافظت</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">کننده عصبی است و پتانسیل ضد دردی آن گزارش شده است. مکانیسم مولکولی داخل مغزی کرایزین در</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">تسکین درد ناشناخته می ­باشد. بنابراین </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">هدف</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">مطالعه حاضر</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">بررسی اثرات</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">کرایزین بر بیان</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">ژن</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">های </span></span></span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Hcrt</span></span></span></i><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black"> و </span></span></span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Pmch</span></span></span></i><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black"> در</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">هیپوتلاموس</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">موش</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">های صحرایی مدل فرمالین می ­باشد.</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">مواد و روش</span></span></span></b><b> </b><b><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">ها: </span></span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">در این مطالعه<b> </b>20 موش صحرایی نر نژاد ویستار به وزن10</span></span></span><span lang="FA" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">±</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">200 گرم به چهار گروه تقسیم شدند (5</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">n=</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">). در بطن سوم مغزی موش ­های صحرایی کانول</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">­</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">گذاری انجام شد. بعد از دوره بهبودی، درد با تزریق زیر پوستی50 میکرولیتر فرمالین 5 درصد به پای عقب مدل حیوان القا شد. موش ­های صحرایی به چهار گروه تقسیم شدند. گروه اول به</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">عنوان گروه کنترل سالم و گروه دوم به</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">عنوان مدل درد القایی با فرمالین سالین ترزیق شد. گروه سوم و چهارم به</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">عنوان</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">گروه­ های درد القایی با فرمالین، به</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">ترتیب 20 و 40 میکروگرم کرایزین را از طریق بطن سوم مغزی دریافت کردند.<b> </b>نمونه ­های هیپوتالاموسی جداسازی و فریز شدند. برای اندازه</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">گیری بیان ژن­ ها، </span></span></span><span dir="LTR" style="font-size:9.0pt"><span new="" roman="" style="font-family:" times="">RT-PCR</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">انجام گرفت.</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">نتایج: </span></span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">درمطالعه حاضر سطح بیان ژن</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">­</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">های </span></span></span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Hcrt</span></span></span></i><b> </b><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">و<b> </b></span></span></span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Pmch</span></span></span></i><b> </b><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">در گروه مدل درد القایی با فرمالین نسبت به گروه کنترل افزایش</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">معنی ­داری یافت. تزریق داخل مغزی کرایزین سبب کاهش معنی ­دار سطح بیان ژن ­های </span></span></span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Hcrt</span></span></span></i><b> </b><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">و<b> </b></span></span></span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Pmch</span></span></span></i><b> </b><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">در مقایسه با گروه مدل درد القایی با فرمالین شد. </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"></span></span></span></span></span></span></span></span><br>
<b><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:"><span style="color:black">بحث و نتیجه</span></span></span></span> <span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:"><span style="color:black">گیری: </span></span></span></span></b><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:"><span style="color:black">در اعمال اثرات ضد دردی کرایزین ممکن</span></span></span></span><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:"><span style="color:black"> است مکانسیم ­های مولکولی داخل هیپوتالاموسی در بالادست محور هیپوتالاموس هیپوفیز آدرنال دخالت داشته باشد.</span></span></span></span></div>
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<td style="padding: 0in 12px; text-align: justify;" valign="top"><span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-family:"Times New Roman",serif"><span style="color:black">Introduction: </span></span></b><span style="font-family:"Times New Roman",serif"><span style="color:black">Chrysin is a flavonoid with neuroprotective effects and its analgesic potential has been reported. The intracerebral molecular mechanism of chrysin in pain relief is unknown. Therefore, the aim of the present study is to investigate the effect of chrysin on</span></span><b> </b><span style="font-family:"Times New Roman",serif"><span style="color:black">hypothalamic<b><i> </i></b><i>Hcrt</i> and <i>Pmch</i></span></span><span style="font-family:"Times New Roman",serif"><span style="color:black"> genes expression in rat model of formalin.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-family:"Times New Roman",serif"><span style="color:black">Materials & Methods:</span></span></b><span style="font-family:"Times New Roman",serif"><span style="color:black"> In this study, 20 male Wistar rats weighing 200±10 g were divided into four groups (n=5). The cannula was implanted in the third cerebral ventricle of rats. </span></span><span style="font-family:"Times New Roman",serif"><span style="color:black">After recovery period, pain was induced by injecting 50 µl formalin into the </span></span><span lang="EN" style="font-family:"Times New Roman",serif"><span style="color:black">hind paw</span></span><span style="font-family:"Times New Roman",serif"><span style="color:black">.</span></span> <span lang="EN" style="font-family:"Times New Roman",serif"><span style="color:black">The group I as control and group II as formalin</span></span><span style="font-family:"Times New Roman",serif"><span style="color:black">-induced pain group</span></span> <span style="font-family:"Times New Roman",serif"><span style="color:black">received saline.</span></span> <span style="font-family:"Times New Roman",serif"><span style="color:black">Groups</span></span><span lang="EN" style="font-family:"Times New Roman",serif"><span style="color:black"> III or IV as a </span></span><span lang="EN" style="font-family:"Times New Roman",serif"><span style="color:black">formalin</span></span><span style="font-family:"Times New Roman",serif"><span style="color:black">-induced pain groups</span></span> <span lang="EN" style="font-family:"Times New Roman",serif"><span style="color:black">received</span></span> <span style="font-family:"Times New Roman",serif"><span style="color:black">20 and 40 μg chrysin via third cerebral ventricle. The hypothalamic samples were removed and frozen. Real-time PCR was performed to measure gene expression. </span></span><span dir="RTL" lang="FA" style="font-family:"Times New Roman",serif"><span style="color:black"></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-family:"Times New Roman",serif"><span style="color:black">Results:</span></span></b><span style="font-family:"Times New Roman",serif"><span style="color:black"> In the present study, genes expression level of </span></span><i><span style="font-family:"Times New Roman",serif"><span style="color:black">Hcrt</span></span></i><span style="font-family:"Times New Roman",serif"><span style="color:black"> and <i>Pmch</i></span></span><span style="font-family:"Times New Roman",serif"><span style="color:black"> increased significantly in the formalin- induced pain group compared to the control. Intracerebral injection of chrysin caused a significant decrease in genes expression level of </span></span><i><span style="font-family:"Times New Roman",serif"><span style="color:black">Hcrt</span></span></i><span style="font-family:"Times New Roman",serif"><span style="color:black"> and <i>Pmch</i></span></span><span style="font-family:"Times New Roman",serif"><span style="color:black"> in comparison to formalin- induced pain group.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-family:"Times New Roman",serif"><span style="color:black">Conclusion:</span></span></b><span style="font-family:"Times New Roman",serif"><span style="color:black"> Intra hypothalamic molecular mechanisms upstream hypothalamus- pituitary- adrenal axis, may be involved in mediating the analgesic effects of chrysin.</span></span><span style="font-family:"Times New Roman",serif"><span style="color:black"></span></span></span></span></span></td>
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Hcrt, درد, فرمالین, کرایزین, Pmch
Hcrt, Pmch, Pain, Formalin, Chrysin
23
28
http://journalaer.com/browse.php?a_code=A-10-2-6&slc_lang=other&sid=1
Khadijeh
Haghighat
خدیجه
حقیقت
10031947532846001297
10031947532846001297
No
Fariba
Mahmoudi
فریبا
محمودی
f.mahmoudi@uma.ac.ir
10031947532846001298
10031947532846001298
Yes
Homayoun
Khazali
همایون
خزعلی
10031947532846001299
10031947532846001299
No