fa بررسی چندشکلی آللی ژن LEPR و ارتباط آن با صفات رشد و لاشه در مرغ بومی مازندران تخصصي Special پژوهشي Research <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">مقدمه:</span></span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">پژوهش حاضر با هدف</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">مطالعه چندشکلی ناحیه اگزون 7 ژن </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">LEPR</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">و ارتباط آن با صفات رشد و لاشه در مرغ بومی مازندران انجام شد.</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black"></span></span></span></span></span></span></span></span></div> <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">مواد و روش &shy;ها:</span></span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">برای این منظور تعداد 200 قطعه جوجه خروس از مرغ</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">های بومی مازندران پرورش داده شده در شرایط یکسان، در سن 12 هفتگی کشتار شدند. صفات مورد بررسی، قبل و بعد از کشتار اندازه</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">گیری و ثبت شدند. قبل از کشتار از تمامی پرندگان نمونه خون تهیه و استخراج </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">DNA</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black"> از نمونه</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">ها با استفاده از کیت شرکت سیناژن صورت گرفت. سپس جایگاه مورد نظر ژن </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">LEPR</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black"> با استفاده از آغازگرهای اختصاصی آن تکثیر و تعیین ژنوتیپ توسط روش </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">PCR-RFLP</span></span></span><span style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black"> با آنزیم اختصاصی </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Bsr</span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">I</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black"> انجام گرفت. تجزیه و تحلیل داده</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">های فنوتیپی و ژنوتیپی با استفاده از نرم</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">افزار آماری </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">9.2</span></span></span> <span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">SAS</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black"> انجام شد.</span></span></span></span></span></span></span></span></div> <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">نتایج:</span></span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">فراوانی هریک از آلل</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">های (+) و (-) در جایگاه مورد نظر ژن </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">LEPR</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">به</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">ترتیب برابر با <span style="color:black">0</span>/675 و <span style="color:black">0</span>/325 برآورد شد. </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">بررسی تعادل هاردی-واینبرگ با استفاده از آزمون کای مربع برای ژن </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">LEPR</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">نشان داد که جمعیت مورد مطالعه در جایگاه ژنی مورد نظر در تعادل نمی</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">باشد. </span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">نتایج نشان داد که ارتباط معنی</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">داری بین ژنوتیپ</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">های ژن </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">LEPR</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">با</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">صفات وزن چربی حفره شکمی، چربی درون عضله</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">ای و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">pH</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black"> گوشت</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">وجود دارد </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">(0/05></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">)</span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">. </span></span></span></span></span></span></span></div> <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">بحث و نتیجه &shy;گیری:</span></span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">براساس نتایج حاصل از این تحقیق می</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">توان نتیجه گرفت که ژن </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">LEPR</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">می</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">تواند در جایگاه مورد نظر به</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">عنوان کاندید برای صفات رشد و لاشه در برنامه</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">های اصلاح نژادی مرغ بومی مازندران مورد استفاده قرار گیرد.</span></span></span></span></span></span></span></div> <div> <table align="center" hspace="0" vspace="0"> <tbody> <tr> <td style="padding: 0in 12px; text-align: justify;" valign="top"><span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-family:&quot;Times New Roman&quot;,serif"><span style="color:black">Introduction:</span></span></b> <span style="font-family:&quot;Times New Roman&quot;,serif">This study aimed to study polymorphism of the exon 7 region of the </span><span style="font-family:&quot;Times New Roman&quot;,serif"><span style="color:black">LEPR</span></span><span style="font-family:&quot;Times New Roman&quot;,serif"> gene and its association with growth and carcass traits in Mazandaran native chicken. </span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-family:&quot;Times New Roman&quot;,serif"><span style="color:black">Materials & Methods:</span></span></b> <span style="font-family:&quot;Times New Roman&quot;,serif">For this purpose, 200 male chicks of Mazandaran native fowls reared in the same conditions and all were slaughtered at 12 weeks of age, were used.</span> <span style="font-family:&quot;Times New Roman&quot;,serif">Traits before and after slaughter were measured and recorded.</span> <span style="font-family:&quot;Times New Roman&quot;,serif">Before the destruction of all birds, blood sample and extraction of DNA from samples <span style="color:#222222">were performed </span>by Cinnagen kit.</span> <span style="font-family:&quot;Times New Roman&quot;,serif"><span style="color:#222222">Then, the desired locus of gene was amplified using specific primers and genotyped using PCR-RFLP method by</span></span><span style="font-family:&quot;Times New Roman&quot;,serif"> <span style="color:black">Bsr</span></span><span style="font-family:&quot;Times New Roman&quot;,serif"><span style="color:black">I</span></span><span style="font-family:&quot;Times New Roman&quot;,serif"><span style="color:#222222"> specific enzyme. </span></span><span style="font-family:&quot;Times New Roman&quot;,serif">Phenotypic and genotypic data analysis conducted using statistical software of SAS9.2. </span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="background:white"><span style="font-family:&quot;Times New Roman&quot;,serif"><span style="color:black">Results:</span></span></span></b>&nbsp;<span style="font-family:&quot;Times New Roman&quot;,serif"><span style="color:#222222">The frequency of each allele (+) and (-) were estimated to be 0.675 and 0.325 in </span></span><span style="font-family:&quot;Times New Roman&quot;,serif"><span style="color:black">LEPR</span></span><span style="font-family:&quot;Times New Roman&quot;,serif"> <span style="color:#222222">locus</span>, respectively.</span> <span style="font-family:&quot;Times New Roman&quot;,serif">Hardy-Weinberg equilibrium study using Chi-square test showed that the study population in loci is not in balance.</span><span style="font-family:&quot;Times New Roman&quot;,serif"> The results </span><span style="font-family:&quot;Times New Roman&quot;,serif">showed a significant association between </span><span style="font-family:&quot;Times New Roman&quot;,serif"><span style="color:black">LEPR</span></span><span style="font-family:&quot;Times New Roman&quot;,serif"> gene genotypes and the traits of </span><span lang="EN" style="font-family:&quot;Times New Roman&quot;,serif">abdominal</span><span style="font-family:&quot;Times New Roman&quot;,serif"> cavity</span><span lang="EN" style="font-family:&quot;Times New Roman&quot;,serif"> fat weight, intramuscular fat and meat pH</span><span style="font-family:&quot;Times New Roman&quot;,serif"> (P<0.05).</span><span dir="RTL" lang="AR-SA" style="background:white"><span style="font-family:&quot;B Nazanin&quot;"><span style="color:black"></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="background:white"><span style="font-family:&quot;Times New Roman&quot;,serif"><span style="color:black">Conclusion:</span></span></span></b> <span style="font-family:&quot;Times New Roman&quot;,serif">Based on the results of this research can be concluded that </span><span style="font-family:&quot;Times New Roman&quot;,serif"><span style="color:black">LEPR</span></span><span style="font-family:&quot;Times New Roman&quot;,serif"> gene can be used as a candidate for growth and carcass traits in Mazandaran native fowls breeding programs.</span></span></span></span></td> </tr> </tbody> </table> </div> <div style="text-align: justify;"></div> ژن, LEPR, چندشکلی, صفات, مرغ بومی Gene, LEPR, Polymorphism, Traits, Native chicken 43 46 http://journalaer.com/browse.php?a_code=A-10-2-10&slc_lang=fa&sid=1 Hossein Attarchi حسین عطارچی hossein_attarchi@yahoo.com 10031947532846001308 10031947532846001308 Yes Mojtaba Tahmoorespoor مجتبی طهمورث پور 10031947532846001309 10031947532846001309 No Mojtaba Ahani Azari مجتبی آهنی آذری 10031947532846001310 10031947532846001310 No Mokhtar Mohajer مختار مهاجر 10031947532846001311 10031947532846001311 No