fa ردیابی مولکولی ویروس آنفلوانزای پرندگان تیپ A در گله های گوشتی دارای علایم تنفسی در برخی استان های ایران عمومى General پژوهشي Research <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">مقدمه‌:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black"> بیماری آنفلوانزای پرندگان یکی از مهم</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">ترین و کشنده</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">ترین بیماری</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">های طیور در سراسر جهان و ایران می</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">باشد که همواره باعث تلفات گسترده در سطح مزارع پرورش طیور می</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">شود. برخی از سویه</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">های ویروس با عبور از سد بین گونه ای پرنده و انسان در گروه بیماری</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">های مشترک یا زئونوز طبقه می</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">شوند. این مطالعه به جهت بررسی نقش ویروس آنفلوانزا در ایجاد تلفات در سطح مزارع پرورش طیور گوشتی کشور طراحی و انجام شده است. بدین جهت تعداد 28 گله از 4 استان مختلف (قزوین، گیلان، زنجان و خوزستان) مورد پایش قرار گرفتند.</span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">مواد و روش &shy;ها:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black"> نمونه</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">ها از تلفات مشکوک به بیماری</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">های تنفسی (از هر گله 10 قطعه مرغ)، و از بافت</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">های نای، ریه، لوزه</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">های سکومی و کلیه</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">ها اخذ شدند. نمونه&shy; بافتی هر 5 قطعه مرغ به</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">صورت</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">جداگانه مخلوط</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">شده و سپس ابتدا با پرایمر</span></span></span> <span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">NP</span></span></span><span style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black"> وجود</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">ویروس آنفلوانزا در بافت</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">ها تایید و در نهایت سروتیپ </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">H9N2</span></span></span><span style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black"> در نمونه</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">های</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">مثبت</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">شده با استفاده از پرایمر اختصاصی </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">H9</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">، ردیابی شد.</span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">نتایج‌:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black"> از مجموع 28 گله پایش شده، ژن </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">NP</span></span></span><span style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black"> ویروس آنفلوانزای نوع </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">A</span></span></span><span style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black"> در 17 گله ردیابی شد و از این 17 گله، در 5 مورد ژن </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">H9</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">&nbsp; مثبت تشخیص داده شدند.</span></span></span></span></span></span></span></span><br> <span class="MsoPlaceholderText" style="color:gray"><b><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:"><span style="color:black">بحث و نتیجه &shy;گیری: </span></span></span></span></b></span><span style="color:#000000;"><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:">با توجه به ارزیابی</span></span></span> <span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:">انجام شده در این مطالعه و شیوع ویروس در تعداد قابل توجهی از مناطق کشور</span></span></span><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:">،</span></span></span><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:"> همواره رعایت اصول بیوسکیوریتی توصیه می</span></span></span> <span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" lotus="" style="font-family:">گردد.</span></span></span></span></div> <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-family:&quot;Times New Roman&quot;,serif">Introduction: </span></b><span style="font-family:&quot;Times New Roman&quot;,serif"><span style="color:black">Avian influenza is one of the most important and deadly poultry diseases all over the world and in Iran, which always causes massive losses in poultry farms. They are classified as common diseases or zoonotic. This study was designed to investigate the role of the influenza virus in causing casualties in the poultry farms of the country. Therefore, 28 flocks from four different provinces (Qazvin, Gilan, Zanjan and Khuzestan) were monitored.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-family:&quot;Times New Roman&quot;,serif">Materials & Methods:</span></b> <span style="font-family:&quot;Times New Roman&quot;,serif">Samples were taken from casualties suspected of respiratory diseases (10 chickens from each flock), and from tissues of the trachea, lungs, cecal tonsils, and kidneys. First, the presence of the influenza virus in the tissues was confirmed with the NP primers, and then the positive samples were evaluated with the specific H9 primer to check the presence of the H9N2 serotype.</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-family:&quot;Times New Roman&quot;,serif">Results:</span></b> <span style="font-family:&quot;Times New Roman&quot;,serif">Out of the total of 28 flocks, 17 flocks were diagnosed positive with the NP primers, and 5 of these 17 flocks were positive with H9-specific primer.</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-family:&quot;Times New Roman&quot;,serif">Conclusion:</span></b> <span style="font-size:12.0pt"><span style="font-family:&quot;Times New Roman&quot;,serif">According to the evaluation done in this study and the prevalence of the virus in a significant number of regions of the country, it is always recommended to follow biosecurity principles.</span></span></span></span></span></div> آنفلوانزای پرندگان, H9N2, علائم تنفسی, گله های گوشتی, آزمون RT-PCR Zinc, In ovo injection, Chicken, Product health, Meat quality 53 58 http://journalaer.com/browse.php?a_code=A-10-1-7&slc_lang=fa&sid=1 Behshad Baheshtian بهشاد بهشتیان B-Beheshtian@stu.scu.ac.ir 10031947532846001370 10031947532846001370 No Zahra Boroomand زهرا برومند z.boroomand@scu.ac.ir 10031947532846001371 10031947532846001371 Yes Masoud Reza Seyfi Abad Shapoori مسعودرضا صیفی آباد شاپوری masoudrs@scu.ac.ir 10031947532846001372 10031947532846001372 No Mansour Mayahi منصور میاحی mansoormayahi@scu.ac.ir 10031947532846001373 10031947532846001373 No