fa بررسی وجود دو ژنوتیپ متفاوت تاس ماهی روسی (Acipenser gueldenstaedtii) بر اساس ناحیه کنترل (دی لوپ) میتوکندریایی در نمونه های تاس ماهیان روسی حوضه جنوبی دریای خزر عمومى General پژوهشي Research <div> <table align="center" hspace="0" vspace="0"> <tbody> <tr> <td style="padding: 0in 12px; text-align: justify;" valign="top"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">مقدمه‌:</span></span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">در این تحقیق، امکان</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">وجود داشتن دو تیپ</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">متفاوت از تاس</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">ماهی روسی براساس توالی ناحیه کنترل (دی</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">لوپ) در</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">نمونه</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">های به</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">دست آمده جغرافیایی حوضه جنوبی دریای خزر، مورد ارزیابی قرار گرفت. هدف ازانجام این تحقیق، ارزیابی وجود یا عدم وجود دو ژنوتیپ مختلف براساس نواحی دی</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">لوپ میتوکندریایی در تاس</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">ماهیان روسی حوضه جنوبی خزر و دستیابی به درصد وجودی هرکدام از این ژنوتیپ</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">ها می</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">باشد. </span></span>&nbsp;</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">مواد و روش &shy;ها:</span></span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">آغازگرهای</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">اختصاصی به شکلی انتخاب شدند تا در صورت امکان بتوان دو ژنوتیپ متفاوت در سطح مولکول </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">DNA</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">میتوکندری</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">شناسایی</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">کرد.</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">نمونه</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">ها از</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">مناطق مختلف</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">جغرافیایی سواحل جنوبی</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">دریای</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">خزر در</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">خلال سال</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">های</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">1381 تا 1400 جمع</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">آوری شده بودند. از روش</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">تکثیر واکنش</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">زنجیره&shy;ای پلی&shy;مرازی</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">چندگانه و </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">DNA</span></span><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"> استخراج شده و اختلاط آغازگرهایی که باندهای هر دو نوع ژنوتیپ را در تاس</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">ماهی روسی تکثیر می&shy; کنند، استفاده شد.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">نتایج‌:</span></span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">نشان داده</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">شد که تاس</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">ماهیان روسی حوضه</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">جنوبی</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">دریای</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">خزر دارای دو</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">نوع ژنوتیپ می</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">باشد</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">که به</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">صورت ژنوتیپ تاس</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">ماهی روسی معمولی با پراکنش 66 درصد و ژنوتیپ شبه تاس</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">ماهی سیبری به</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">میزان 28 درصد تعریف می</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">شوند</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">که ژنوتیپ شبه</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">تاس</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">ماهی سیبری از پراکنش کم</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">تری در بین نمونه</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:">های مورد بررسی برخوردار بود.</span></span></span></span></span></span></span><br> <span class="MsoPlaceholderText" style="color:gray"><b><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:"><span style="color:black">بحث تیجه&shy; گیری: </span></span></span></span></b></span><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:">در این روش</span></span></span> <span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:">شناسایی، می&shy; توان</span></span></span> <span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:">کار بررسی</span></span></span> <span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:">دو ژنوتیپ مختلف تاس</span></span></span> <span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:">ماهی روسی را در</span></span></span> <span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:">مدت</span></span></span> <span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:">زمان</span></span></span> <span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:">کوتاهی انجام داد که در مطالعات تعیین هویتی و بررسی جمعیتی برای تاس</span></span></span> <span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:">ماهی روسی کاربرد خواهد داشت.</span></span></span></td> </tr> </tbody> </table> </div> <div style="text-align: justify;"></div> <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-family:&quot;Times New Roman&quot;,serif">Introduction: </span></b><span style="font-family:&quot;Times New Roman&quot;,serif">In this study, the potential existence of two distinct types of Russian sturgeon was evaluated based on the sequence of the control region (D-loop) in samples obtained from the southern Caspian Sea basin. The aim of this research was to assess the presence or absence of two mitochondrial D-loop genotypes in Russian sturgeon of the southern Caspian basin and to determine the frequency of each genotype. </span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-family:&quot;Times New Roman&quot;,serif">Materials & Methods:</span></b> <span style="font-family:&quot;Times New Roman&quot;,serif">Specific primers were designed in such a way as to enable the identification of two different genotypes at the mitochondrial DNA level, if present. Samples were collected from various geographical regions along the southern Caspian coasts countries during the years 2002 to 2021. Using multiplex polymerase chain reaction (PCR) with extracted DNA and a mixture of primers capable of amplifying the bands of both genotypes in Russian sturgeon.</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-family:&quot;Times New Roman&quot;,serif">Results:</span></b> <span style="font-family:&quot;Times New Roman&quot;,serif">it was demonstrated that Russian sturgeons of the southern Caspian basin possess two mitochondrial genotypes. These were defined as the &ldquo;typical Russian sturgeon genotype,&rdquo; with a frequency of 66%, and the &ldquo;Siberian-like genotype,&rdquo; with a frequency of 28%. The Siberian-like genotype occurred less frequently among the examined samples. </span></span></span></span><br> <b><span style="font-size:11.0pt"><span style="line-height:107%"><span style="font-family:&quot;Times New Roman&quot;,serif">Conclusion:</span></span></span></b> <span style="font-size:11.0pt"><span style="line-height:107%"><span style="font-family:&quot;Times New Roman&quot;,serif">This identification method allows for the rapid detection of the two genotypes of Russian sturgeon and can be effectively applied in identity determination and population studies of this species.</span></span></span></div> تاس ماهی روسی (Acipenser gueldenstaedtii), حوضه جنوبی دریای خزر, میتوکندری, دی لوپ, ژنوتیپ Russian sturgeon, (Acipenser gueldenstaedti), Southern Caspian Sea Basin, Mitochondrion, Genotype 69 74 http://journalaer.com/browse.php?a_code=A-10-1-10&slc_lang=fa&sid=1 Shirin Jamshidi شیرین جمشیدی 10031947532846001378 10031947532846001378 Yes