fa بررسی تنوع ریزجلیک ها با تاکید بر شناسایی مولکولی و ارزش تجاری دو جنس Haematococcus و Scendesmus در سد کلوانس (خوی-ایران) تخصصي Special پژوهشي Research <div> <table align="center" hspace="0" vspace="0"> <tbody> <tr> <td style="padding: 0in 12px; text-align: justify;" valign="top"><span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">مقدمه:</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black"> <span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span b="" baran="" style="font-family:"><span style="font-style:normal">ریزجلبک &shy;ها به</span></span></span></span></span></span></span></span></span> <span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal">دلیل داشتن کلروفیل موجودات تولیدکننده به</span></span></span></span></span></span></span> <span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal">حساب می &shy;آیند و غنی بودن آن</span></span></span></span></span></span></span> <span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal">ها از اسید&shy;های چرب و رنگیزه&shy;های مختلف، به کاربرد آن</span></span></span></span></span></span></span> <span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal">ها تنوع بخشیده و به اهمیت بررسی آن</span></span></span></span></span></span></span> <span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal">ها از دیدگاه اکولوژیک و بیوتکنولوژیک افزوده است. این تحقیق با هدف بررسی کیفیت آب، تنوع ریز&shy;جلبک&shy; ها و امکان تجاری&shy; سازی گونه &shy;های مهم ریز&shy;جلبک انجام گرفت. &nbsp;</span></span></span></span></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">مواد و روش</span></span></span></b><b> </b><b><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">ها:</span></span></span></b> <span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal">در این تحقیق، نمونه &shy;برداری از آب سد</span></span></span></span></span></span></span> <span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal">کلوانس واقع در آذربایجان</span></span></span></span></span></span></span> <span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal">غربی در4 ایستگاه انجام شد. کشت نمونه &shy;های جلبک در محیط </span></span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt"><span style="font-style:normal">Walne</span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal"> صورت گرفته و شناسایی ریز جلبک&shy; ها با استفاده از</span></span></span></span></span></span></span> <span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal">کلیدهای شناسایی صورت پذیرفت. به</span></span></span></span></span></span></span> <span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal">علت شباهت زیاد برخی از گونه &shy;های ریز&shy;جلبک به یکدیگر، استفاده از روش &shy;های</span></span></span></span></span></span></span> <span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal">مولکولی به</span></span></span></span></span></span></span> <span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal">منظور شناسایی دقیق ریز&shy;جلبک &shy;ها در سطح گونه، مورد توجه قرار گرفت. دوگونه تجاری متعلق به خانواده </span></span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt">Haematococcaceae</span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal"> و </span></span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt">Scendesmaceae</span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal"> از بین این جلبک &shy;ها ایزوله شده، با تکنیک&shy; های</span></span></span></span></span></span></span> <span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal">مولکولی با استفاده از توالی</span></span></span></span></span></span></span> <span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal">یابی ناحیه </span></span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt"><span style="font-style:normal">ITS</span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal"> شناسایی شده و پروفایل اسیدهای چرب آن</span></span></span></span></span></span></span> <span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal">ها مورد بررسی واقع شد.</span></span></span></span></span></span></span><br> <b><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">نتایج:</span></span></span></b> <span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal">بررسی اولیه موفق به شناسایی چندین جنس و گونه شامل </span></span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt">Scendesmus</span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt"><span style="font-style:normal"> sp.</span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal">، </span></span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt">Haematococcus</span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt"><span style="font-style:normal"> sp.</span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal">،</span></span></span></span></span></span></span> <span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt">Navicula</span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt"><span style="font-style:normal"> sp.</span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal">، </span></span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt">Pandorina</span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt"><span style="font-style:normal"> sp</span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal">، </span></span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt">Scendesmus</span></span></span></span></span> <span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt">dimorpha</span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal"> و </span></span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt">Pediastrum</span></span></span></span></span> <span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt">boryanum</span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal"> گردید. پس از تجزیه و تحلیل مولکولی توالی مربوط به گونه&shy; های </span></span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt">Haematococcus</span></span></span></span></span> <span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt">lacustris</span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal"> و </span></span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt">Scendesmus</span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt"><span style="font-style:normal">sp.</span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal"> در بانک جهانی ژن یا </span></span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt"><span style="font-style:normal">NCBI</span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal"> با کد </span></span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt"><span style="font-style:normal">ON975001.0</span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal"> و </span></span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt"><span style="font-style:normal">ON955512.1</span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal"> ثبت شده و این گونه &shy;ها به &shy;طور دقیق شناسایی شدند. بررسی پروفایل اسیدهای چرب این دو گونه نشان داد که این جلبک &shy;ها ارزش غذایی بالایی برای استفاده در صنایع مختلف را دارند. هم</span></span></span></span></span></span></span> <span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal">چنین، در این تحقیق، استفاده از نشانگر </span></span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt"><span style="font-style:normal">ITS</span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal"> به عنوان روشی دقیق و مطمئن در شناسایی گونه &shy;های ریزجلبک پیشنهاد شد.</span></span></span></span></span></span></span></span></span></span></span></span> <div><b><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:"><span style="color:black">بحث و نتیجه</span></span></span></span> <span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:"><span style="color:black">گیری: </span></span></span></span></b><span class="fontstyle11" new="" roman="" style="font-family: " times=""><span style="color:black"><span style="font-style:italic"><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:"><span style="font-style:normal">بررسی خصوصیات فیزیکوشیمیایی آب سد کلوانس نیز نشان داد که آب منطقه با رعایت اصول زیست محیطی توانایی ذخیره&shy; سازی لارو آبزیان و پرورش انبوه ریزجلبک &shy;ها را دارد.</span></span></span></span></span></span></span></div> </td> </tr> </tbody> </table> </div> <div style="text-align: justify;"></div> <div style="text-align: justify;"><span style="font-size:11pt"><span style="background:white"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-family:&quot;Times New Roman&quot;,serif"><span style="color:black">Introduction:</span></span></b> <span style="font-family:&quot;Times New Roman&quot;,serif"><span style="color:black">Microalgae are considered productive organisms due to their chlorophyll and their richness in fatty acids and various pigments has diversified their use and increased the importance of their investigation from an ecological and biotechnological point of view. This study aimed to investigate water quality, diversity of microalgae, and the possibility of commercializing important microalgae species. </span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="background:white"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-family:&quot;Times New Roman&quot;,serif"><span style="color:black">Materials & Methods:</span></span></b> <span style="background:white"><span style="font-family:&quot;Times New Roman&quot;,serif"><span style="color:black">Sampling was carried out from the Kelvans Dam located in West Azerbaijan at 4 stations. The algae samples were cultured in a Walne medium, and the identification of microalgae was performed using identification keys. Due to the high similarity of some microalgae species, molecular methods were used to accurately identify microalgae at the species level. Two commercially important species belonging to the Haematococcaceae and Scendesmaceae families were isolated among these algae, identified using molecular techniques through ITS sequencing, and their fatty acid profiles were examined. </span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-family:&quot;Times New Roman&quot;,serif"><span style="color:black">Results:</span></span></b> <span style="background:white"><span style="font-family:&quot;Times New Roman&quot;,serif"><span style="color:black">The initial investigation successfully identified several genera and species including <i>Scendesmus</i> sp., <i>Haematococcus</i> sp., <i>Navicula</i> sp., <i>Pandorina</i> sp., <i>Scendesmus</i> <i>dimorpha</i>, and <i>Pediastrum</i> <i>boryanum</i>. After molecular analysis, the sequences related to <i>Haematococcus lacustris</i> and <i>Scendesmus</i> sp. were recorded in the NCBI GenBank with codes ON975001.0 and ON955512.1, and these species were precisely identified. The examination of the fatty acid profiles of these two species showed that they have high nutritional value for use in various industries. Additionally, in this study, the use of ITS marker as an accurate and reliable method for identifying microalgae species was suggested.</span></span></span></span></span></span></span></span><br> <b><span style="font-size:11.0pt"><span style="line-height:107%"><span style="font-family:&quot;Times New Roman&quot;,serif"><span style="color:black">Conclusion:</span></span></span></span></b> <span style="font-size:11.0pt"><span style="line-height:107%"><span style="font-family:&quot;Times New Roman&quot;,serif"><span style="color:black">The physicochemical characteristics of the Kelvans Dam water also showed that the water of the region has the potential to store fish larvae and mass-cultivate microalgae while adhering to environmental principles.</span></span></span></span></div> جلبک های تک سلولی, اسیدهای چرب, آذربایجان غربی, نشانگر ITS, ارزش غذایی Unicellular algae, Fatty acids, West Azerbaijan, ITS marker, Nutritional value 141 150 http://journalaer.com/browse.php?a_code=A-10-2-32&slc_lang=fa&sid=1 Sakineh Moradkhani سکینه مرادخانی 10031947532846001410 10031947532846001410 No Ramin Manaffar رامین مناف فر r.manaffar@urmia.ac.ir 10031947532846001411 10031947532846001411 Yes Vahid Ghasemlou وحید قاسملو 10031947532846001412 10031947532846001412 No Hediyeh Yazdani هدیه یزدانی 10031947532846001413 10031947532846001413 No