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:: دوره 1، شماره 4 - ( 10-1404 ) ::
جلد 1 شماره 4 صفحات 104-93 برگشت به فهرست نسخه ها
شناسایی جایگاه های ژنومی مرتبط با صفت رسیدگی جنسی ماهی قزل آلای رنگین کمان (Oncorhyncus mykiss) با استفاده از تجزیه و تحلیل بیوانفورماتیکی
سلطنت نجارلشگری* ، سجاد نظری ، شیرین جمشیدی
چکیده:   (990 مشاهده)
مقدمه: روش تجزیه و تحلیل بیوانفورماتیکی مناطق ژنومی در مطالعات نقشه ­یابی صفات کمی از اهمیت زیادی برخوردار می­ باشد. این مطالعه با هدف شناسایی جایگاه ­های ژنومی مرتبط با صفت رسیدگی جنسی ماهی قزل ­آلای رنگین­ کمان به ­روش فراتحلیل داده ­ها انجام شد.
مواد و روش ها: بدین منظور اطلاعات ژنوم ماهی قزل ­آلای رنگین­ کمان برای شناسایی جایگاه­ های صفات کمی در بانک پایگاه­ های اطلاعاتی مورد تجزیه و تحلیل قرار گرفت. سپس اطلاعات مربوط به نرم­ افزارهای آماری مورد استفاده در نقشه ­یابی ارتباطی ارائه و پس از آن فرصت­ ها و چالش ­های نقشه ­یابی ارتباطی و مطالعات پسا نقشه ­یابی در سطح کل ژنوم مورد بحث قرار گرفتند. برای نقشه ­یابی جایگاه­ های صفات کمی مرتبط با یک نشانگر مولکولی، توالی نوکلئوتیدی آن نشانگر و برای نقشه ­یابی جایگاه­ های صفات کمی مرتبط با دو نشانگر مولکولی مجاور، توالی نوکلئوتیدی هر دو نشانگر مجاور یکدیگر از پایگاه ­های اطلاعاتی مربوطه استخراج شد. مکان ­یابی هر جایگاه صفت کمی براساس نقشه ژنتیکی و در سطح اطمینان 95 % انجام و از نرم­افزار Bio Mercator برای پیش ­بینی مکان و سطح اطمینان MQTL براساس روش حداکثر احتمال استفاده شد.
نتایج: در مجموع تعداد 11 MQTL ارتباط معنی ­داری را نشان دادند و بیش ترین تعداد جایگاه­ های صفات کمی شناسایی شده در گروه پیوستگی 6، 8، 12 و 13 قرار داشتند.
بحث و نتیجه گیری: نتایج نشان داد که جایگاه ­های صفات کمی هم مکان برای صفت مورد مطالعه وجود دارند که می ­توانند در به ­نژادی و مهندسی ژنتیک ماهی قزل ­آلای رنگین ­کمان به کار روند.
واژه‌های کلیدی: بیوانفورماتیک، ژنوم، رسیدگی جنسی، قزل آلای رنگین کمان، QTL
متن کامل [PDF 1110 kb]   (288 دریافت)    
نوع مطالعه: مروری | موضوع مقاله: تخصصي
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Najjar Lashgari S, Nazari S, Jamshidi S. Identification of genomic loci related to the sexual maturation trait of rainbow trout using bioinformatics analysis. , Journalaer. 2025; 1 (4) :93-104
URL: http://journalaer.com/article-1-56-fa.html

نجارلشگری سلطنت، نظری سجاد، جمشیدی شیرین. شناسایی جایگاه های ژنومی مرتبط با صفت رسیدگی جنسی ماهی قزل آلای رنگین کمان (Oncorhyncus mykiss) با استفاده از تجزیه و تحلیل بیوانفورماتیکی. , پژوهش های محیط زیست جانوری. 1404; 1 (4) :93-104

URL: http://journalaer.com/article-1-56-fa.html



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دوره 1، شماره 4 - ( 10-1404 ) برگشت به فهرست نسخه ها
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