fa بررسی تنوع ریزجلیک ها با تاکید بر شناسایی مولکولی و ارزش تجاری دو جنس Haematococcus و Scendesmus در سد کلوانس (خوی-ایران) تخصصي Special پژوهشي Research <div> <table align="center" hspace="0" vspace="0"> <tbody> <tr> <td style="padding: 0in 12px; text-align: justify;" valign="top"><span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">مقدمه:</span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black"> <span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span b="" baran="" style="font-family:"><span style="font-style:normal">ریزجلبک &shy;ها به</span></span></span></span></span></span></span></span></span> <span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal">دلیل داشتن کلروفیل موجودات تولیدکننده به</span></span></span></span></span></span></span> <span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal">حساب می &shy;آیند و غنی بودن آن</span></span></span></span></span></span></span> <span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal">ها از اسید&shy;های چرب و رنگیزه&shy;های مختلف، به کاربرد آن</span></span></span></span></span></span></span> <span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal">ها تنوع بخشیده و به اهمیت بررسی آن</span></span></span></span></span></span></span> <span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal">ها از دیدگاه اکولوژیک و بیوتکنولوژیک افزوده است. این تحقیق با هدف بررسی کیفیت آب، تنوع ریز&shy;جلبک&shy; ها و امکان تجاری&shy; سازی گونه &shy;های مهم ریز&shy;جلبک انجام گرفت. &nbsp;</span></span></span></span></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:18.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">مواد و روش</span></span></span></b><b> </b><b><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">ها:</span></span></span></b> <span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal">در این تحقیق، نمونه &shy;برداری از آب سد</span></span></span></span></span></span></span> <span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal">کلوانس واقع در آذربایجان</span></span></span></span></span></span></span> <span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal">غربی در4 ایستگاه انجام شد. کشت نمونه &shy;های جلبک در محیط </span></span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt"><span style="font-style:normal">Walne</span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal"> صورت گرفته و شناسایی ریز جلبک&shy; ها با استفاده از</span></span></span></span></span></span></span> <span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal">کلیدهای شناسایی صورت پذیرفت. به</span></span></span></span></span></span></span> <span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal">علت شباهت زیاد برخی از گونه &shy;های ریز&shy;جلبک به یکدیگر، استفاده از روش &shy;های</span></span></span></span></span></span></span> <span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal">مولکولی به</span></span></span></span></span></span></span> <span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal">منظور شناسایی دقیق ریز&shy;جلبک &shy;ها در سطح گونه، مورد توجه قرار گرفت. دوگونه تجاری متعلق به خانواده </span></span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt">Haematococcaceae</span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal"> و </span></span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt">Scendesmaceae</span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal"> از بین این جلبک &shy;ها ایزوله شده، با تکنیک&shy; های</span></span></span></span></span></span></span> <span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal">مولکولی با استفاده از توالی</span></span></span></span></span></span></span> <span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal">یابی ناحیه </span></span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt"><span style="font-style:normal">ITS</span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal"> شناسایی شده و پروفایل اسیدهای چرب آن</span></span></span></span></span></span></span> <span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal">ها مورد بررسی واقع شد.</span></span></span></span></span></span></span><br> <b><span lang="FA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="color:black">نتایج:</span></span></span></b> <span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal">بررسی اولیه موفق به شناسایی چندین جنس و گونه شامل </span></span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt">Scendesmus</span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt"><span style="font-style:normal"> sp.</span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal">، </span></span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt">Haematococcus</span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt"><span style="font-style:normal"> sp.</span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal">،</span></span></span></span></span></span></span> <span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt">Navicula</span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt"><span style="font-style:normal"> sp.</span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal">، </span></span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt">Pandorina</span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt"><span style="font-style:normal"> sp</span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal">، </span></span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt">Scendesmus</span></span></span></span></span> <span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt">dimorpha</span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal"> و </span></span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt">Pediastrum</span></span></span></span></span> <span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt">boryanum</span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal"> گردید. پس از تجزیه و تحلیل مولکولی توالی مربوط به گونه&shy; های </span></span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt">Haematococcus</span></span></span></span></span> <span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt">lacustris</span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal"> و </span></span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt">Scendesmus</span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt"><span style="font-style:normal">sp.</span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal"> در بانک جهانی ژن یا </span></span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt"><span style="font-style:normal">NCBI</span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal"> با کد </span></span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt"><span style="font-style:normal">ON975001.0</span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal"> و </span></span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt"><span style="font-style:normal">ON955512.1</span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal"> ثبت شده و این گونه &shy;ها به &shy;طور دقیق شناسایی شدند. بررسی پروفایل اسیدهای چرب این دو گونه نشان داد که این جلبک &shy;ها ارزش غذایی بالایی برای استفاده در صنایع مختلف را دارند. هم</span></span></span></span></span></span></span> <span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal">چنین، در این تحقیق، استفاده از نشانگر </span></span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span dir="LTR" style="font-size:10.0pt"><span style="font-style:normal">ITS</span></span></span></span></span></span><span class="fontstyle11" new="" roman="" style="font-family:" times=""><span style="color:black"><span style="font-weight:normal"><span style="font-style:italic"><span lang="AR-SA" style="font-size:12.0pt"><span b="" baran="" style="font-family:"><span style="font-style:normal"> به عنوان روشی دقیق و مطمئن در شناسایی گونه &shy;های ریزجلبک پیشنهاد شد.</span></span></span></span></span></span></span></span></span></span></span></span> <div><b><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:"><span style="color:black">بحث و نتیجه</span></span></span></span> <span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:"><span style="color:black">گیری: </span></span></span></span></b><span class="fontstyle11" new="" roman="" style="font-family: " times=""><span style="color:black"><span style="font-style:italic"><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" baran="" style="font-family:"><span style="font-style:normal">بررسی خصوصیات فیزیکوشیمیایی آب سد کلوانس نیز نشان داد که آب منطقه با رعایت اصول زیست محیطی توانایی ذخیره&shy; سازی لارو آبزیان و پرورش انبوه ریزجلبک &shy;ها را دارد.</span></span></span></span></span></span></span></div> </td> </tr> </tbody> </table> </div> <div style="text-align: justify;"></div> جلبک های تک سلولی, اسیدهای چرب, آذربایجان غربی, نشانگر ITS, ارزش غذایی 141 150 http://journalaer.com/browse.php?a_code=A-10-2-32&slc_lang=fa&sid=1 Sakineh Moradkhani سکینه مرادخانی 1003194753284600392 1003194753284600392 No Ramin Manaffar رامین مناف فر r.manaffar@urmia.ac.ir 1003194753284600393 1003194753284600393 Yes Vahid Ghasemlou وحید قاسملو 1003194753284600394 1003194753284600394 No Hediyeh Yazdani هدیه یزدانی 1003194753284600395 1003194753284600395 No