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Investigation of the cytotoxic effects of the measles virus matrix gene on the SW480 colorectal cancer cells
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Solmaz Morovati   , Ali Mohammadi * , Amir Ali Heidari , Mehdi Asad Sangabi |
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Abstract: (267 Views) |
Introduction: Colorectal cancer is a highly prevalent malignant disease affecting both men and women globally. colorectal cancer typically demonstrates resistance to conventional therapies. This characteristic renders it a promising contender for the implementation of alternative approaches such as onco-virotherapy. These strategies aim to overcome the limitations posed by traditional treatments. Oncolytic viruses, including measles virus, have been extensively studied in therapeutic models and clinical phases. Nevertheless, there is still a need to fully comprehend the precise mechanism and significance of individual viral proteins in the induction of cell death by the virus.
Materials & Methods: This study presents the initial exploration of the cytotoxic impact of the measles virus’ matrix gene on human SW480 colorectal cancer cells. The SW480 cells were individually transfected with pcDNA3 plasmids containing the matrix gene of the measles virus AIK-C attenuated strain. The recombinant plasmids were verified using agarose gel electrophoresis. Then the MTT assay was employed to confirm the cytotoxic effects of the virus’ matrix gene on colorectal cancer cells.
Results: The findings revealed that this gene induced a reduction in cell viability, affecting approximately 86.5% of the cells at the 96-hour post-transfection time point.
Conclusion: In conclusion, the results suggest that the matrix gene of the measles virus has the potential to serve as a targeted therapy for cancers. |
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| Keywords: Colorectal cancer, Oncolytic viruses, Matrix, Measles, pcDNA3 plasmid |
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Full-Text [PDF 699 kb]
(79 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2026/05/27 | Accepted: 2025/08/1 | Published: 2025/08/1
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