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Analysis of the Effects of Sargassum tenerrimum Algal Extract on Interleukin-12 Gene Expression in Lymphoblastic Leukemia C121 Cells
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Maryam Nourniaei   , Hoda Khaledi * , Ali Khodadadi , Seyedeh Maryam Mousavi , Rezvan Attari |
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Abstract: (271 Views) |
Introduction: Leukemia is the fifth most common cancer in the world. In addition to existing chemotherapy drugs, attention has been drawn to natural resources and their constituents for the treatment of various cancers. One of these resources is marine algae, which are rich in biologically active compounds with various functions. Therefore, the aim of the present study was to investigate the effect of Sargassum tenerrimum extract on the expression of the interleukin-12 (IL-12) gene in C121 cell line.
Materials & Methods: For this purpose, after sampling the algae in the spring from the intertidal zone of the coasts of Bushehr, transferring them to the laboratory and morphological identification of the algae, extraction was performed using methanol, chloroform and ethanol solvents. C121 cells were cultured in RPMI 1640 culture medium and treated with concentrations of 250, 500 and 750 micrograms per milliliter of Sargassum tenerrimum extract for 24 hours. After this time, the RNA of the cells was extracted and the expression of the interleukin-12 (IL-12) gene was evaluated using the Real-Time PCR method.
Results: The results showed that the expression of the IL-12 gene in the C121 cell line treated with all the studied concentrations (250, 500 and 750 micrograms per milliliter) of chloroform, hydroalcoholic and methanolic extracts of Sargassum tenerrimum was increased compared to the control group. The greatest effect on increasing the expression of this gene was observed in the concentrations of 500 micrograms per milliliter of chloroform and methanolic extract (p<0.05). The findings suggest that the type and concentration of the extract can have a significant effect on the IL-12-mediated immune response.
Conclusion: Based on the increase in IL-12 expression under the effect of Sargassum tenerrimum extract, it can be concluded that the extract of this alga has anti-cancer potential against lymphoblastic leukemia. Further research to find the active ingredient present in the extract and also to understand the mechanisms involved in it can help to realize its potential anti-cancer potential. |
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| Keywords: Marine algae, Anti-cancer, Persian Gulf, Marine extract, Real-Time PCR |
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Full-Text [PDF 936 kb]
(72 Downloads)
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Type of Study: Research |
Subject:
Special
Received: 2026/05/27 | Accepted: 2025/08/1 | Published: 2025/08/1
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