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Investigating the effects of chrysin on hypothalamic orexin and melanin-concentrating hormone gene expression in a rat model of formalin- induced pain
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Khadijeh Haghighat   , Fariba Mahmoudi * , Homayoun Khazali |
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Abstract: (996 Views) |
Introduction: Chrysin is a flavonoid with neuroprotective effects and its analgesic potential has been reported. The intracerebral molecular mechanism of chrysin in pain relief is unknown. Therefore, the aim of the present study is to investigate the effect of chrysin on hypothalamic Hcrt and Pmch genes expression in rat model of formalin.
Materials & Methods: In this study, 20 male Wistar rats weighing 200±10 g were divided into four groups (n=5). The cannula was implanted in the third cerebral ventricle of rats. After recovery period, pain was induced by injecting 50 µl formalin into the hind paw. The group I as control and group II as formalin-induced pain group received saline. Groups III or IV as a formalin-induced pain groups received 20 and 40 μg chrysin via third cerebral ventricle. The hypothalamic samples were removed and frozen. Real-time PCR was performed to measure gene expression.
Results: In the present study, genes expression level of Hcrt and Pmch increased significantly in the formalin- induced pain group compared to the control. Intracerebral injection of chrysin caused a significant decrease in genes expression level of Hcrt and Pmch in comparison to formalin- induced pain group.
Conclusion: Intra hypothalamic molecular mechanisms upstream hypothalamus- pituitary- adrenal axis, may be involved in mediating the analgesic effects of chrysin. |
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| Keywords: Hcrt, Pmch, Pain, Formalin, Chrysin |
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Full-Text [PDF 797 kb]
(312 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2025/08/24 | Accepted: 2025/10/2 | Published: 2025/10/2
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