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Investigation of the Existence of Two Different Genotypes of Russian Sturgeon (Acipenser gueldenstaedtii) Based on the Mitochondrial Control Region (D-loop) in Samples from the Southern Caspian Sea Basin
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Shirin Jamshidi *   |
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Abstract: (750 Views) |
Introduction: In this study, the potential existence of two distinct types of Russian sturgeon was evaluated based on the sequence of the control region (D-loop) in samples obtained from the southern Caspian Sea basin. The aim of this research was to assess the presence or absence of two mitochondrial D-loop genotypes in Russian sturgeon of the southern Caspian basin and to determine the frequency of each genotype.
Materials & Methods: Specific primers were designed in such a way as to enable the identification of two different genotypes at the mitochondrial DNA level, if present. Samples were collected from various geographical regions along the southern Caspian coasts countries during the years 2002 to 2021. Using multiplex polymerase chain reaction (PCR) with extracted DNA and a mixture of primers capable of amplifying the bands of both genotypes in Russian sturgeon.
Results: it was demonstrated that Russian sturgeons of the southern Caspian basin possess two mitochondrial genotypes. These were defined as the “typical Russian sturgeon genotype,” with a frequency of 66%, and the “Siberian-like genotype,” with a frequency of 28%. The Siberian-like genotype occurred less frequently among the examined samples.
Conclusion: This identification method allows for the rapid detection of the two genotypes of Russian sturgeon and can be effectively applied in identity determination and population studies of this species. |
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| Keywords: Russian sturgeon, (Acipenser gueldenstaedti), Southern Caspian Sea Basin, Mitochondrion, Genotype |
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Full-Text [PDF 621 kb]
(243 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2025/10/11 | Accepted: 2025/09/1 | Published: 2025/12/2
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