|
Investigating the effect of GABA on the biomass and the expression of some genes involved in the astaxanthin biosynthesis pathway of Haematococcus pluvialis under different light conditions
|
Ahmad Ghasemi *   , Gholamreza Abdi , Afshin Jokar |
|
|
|
Abstract: (782 Views) |
Introduction: Green microalgae contain a wide array of valuable metabolites that can potentially attract numerous biomanufacturing businesses. Among these microalgae, Haematococcus pluvialis, a unicellular freshwater microalga belonging to the Chlorophyceae class, is prevalent in numerous aquatic environments worldwide. It has gained recognition as the most abundant and auspicious reservoir for the industrial-scale synthesis of natural astaxanthin. The effects of γ-aminobutyric acid (GABA) (0 and 0.0001 g/L) on Haematococcus pluvialis different light condition were investigated in order to find the best inducer and suitable light type (white, blue, and red light) for maximizing the photosynthesis efficiency and low-cost production.
Materials & Methods: Microalgal cells, after growth phase, were subjected to the different treatments and cell growth rate, chlorophyll, carotenoids, total astaxanthin content, and the expression of some involved genes in astaxanthin biosynthesis pathways (psy, lcy, bkt2) were measured.
Results: The results showed that the blue light increased cell division and growth efficiency (2.64-fold) and red light increased biosynthesis and accumulation of astaxanthin (94.4 mg/g). Additionally, using GABA enhanced the cell growth and astaxanthin production. GABA under red light resulted to a significantly higher accumulation of astaxanthin compared to white and blue light, although it had a lower cell growth rate and division. Gene expression results also indicated that GABA significantly induces psy and pds genes under blue and red light.
Conclusion: Collectively, our findings offer a coordinated approach to enhance the coproduction of astaxanthin by using the combination of red illuminate and GABA which influences on cell proliferation and astaxanthin synthesis in H. pluvialis. |
|
| Keywords: GABA, Melatonin, Astaxanthin, Fatty acids, Haematococcus pluvialis, Different light conditions, Gene expression |
|
|
Full-Text [PDF 978 kb]
(259 Downloads)
|
Type of Study: Research |
Subject:
Special
Received: 2025/11/3 | Accepted: 2025/09/1 | Published: 2025/12/2
|
|
|
|
|
|
|
| Send email to the article author |
|
|
|
| Add your comments about this article |
|
|
|
