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Identification and diagnosis of viral hemorrhagic septicemia (VHS) in Rainbow trout (Oncorhynchus mykiss) farms in Tehran province
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Mohammad Hadi Daneshi *   |
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Abstract: (816 Views) |
Introduction: In Iran, Viral Hemorrhagic Septicemia (VHS) is one of the main causes of mortality of farmed trout in recent years. The use of the Real-Time PCR method will be significant in controlling and preventing rainbow trout viral diseases in the country. Therefore, this research investigated the identification and diagnosis of VHSV in rainbow trout farms in Tehran province.
Materials & Methods: In this study, which was conducted in two phases, winter and spring 2022-3 and winter and spring 2023-4, by referring to rainbow trout breeding farms in Tehran province, samples were taken from fry weighing 0.3-3 was heated and using Quantitative Real-Time PCR method to identify and diagnose VHSV in rainbow trout farms in Tehran province.
Results: According to the obtained results, VHS viral disease had the highest multiplication rate in the spring of 2024 in the fields and had a significant difference. In the results obtained in the winter of 2022 and the spring of 2023, the percentage of virus detection in breeding farms was the same, and these results were equal to the percentage of virus prevalence in the winter of 2024, and no significant difference was observed. According to the summary of the above results, the prevalence of the disease in Tehran province was reported as 9.8%.
Conclusion: In the first three months of 2024, the disease had a clear and higher prevalence than last year, which is due to the increase of the disease in Takhir farms and needs more attention to improve biosecurity and disease control. VHS virus in circulation in the country depends on the weight, and often, the clinical form of disease and death does not occur in baby fish above 2 grams. Therefore, it seems that to establish an acceptable level of biosecurity system at the level of these farms, in addition to the necessary training for growers, more effective measures should be planned and implemented. |
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| Keywords: Viral hemorrhagic septicemia (VHS), Rainbow trout, Real-time PCR, Tehran province |
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Full-Text [PDF 635 kb]
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Type of Study: Review |
Subject:
Special
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1. Oie. 2017. Manual of diagnostic tests for aquatic animals. Chapter 2.3. 10. Viral haemorrhagic septicaemia. 25 p. 2. LaPatra, S., Misk, E., Al-Hussinee, L. and Lumsden, J.S., 2024. Rhabdoviruses of fish. In Kibenge, F.S.B. and Godoy, M.G., (Eds.). Aquaculture Virology (Second Edition). Chapter 20. Academic Press. 315 p. https://doi.org/10.1016/B978-0-323-91169-6.00048-0 [ DOI] 3. Jang, M.S., Oh, M.J. and Kim, W.S., 2024. Evaluation of enzyme-linked immunosorbent assay (ELISA) for detection of olive flounder antibodies to viral hemorrhagic septicemia virus (VHSV, genotype IVa) using two Novirhabdovirus antigens. Journal of fish pathology. 37(1): 9-15. http://dx.doi.org/10.7847/jfp.2024.37.1.009 [ DOI] 4. Ahmadivand, S., Soltani, M., Mardani, K., Shokrpoor, S., Rahmati-Holasoo, H., Mokhtari, A. and Hasanzadeh, R., 2016. Isolation and identification of viral hemorrhagic septicemia virus (VHSV) from farmed rainbow trout (Oncorhynchus mykiss) in Iran. Acta tropica. 156: 30-36. doi: 10.1016/j.actatropica.2016.01.005 [ DOI] 5. Mohammadisefat, P., Zorriehzahra, M.J., Adel, M., Allahbeygi Chamjangali, Z., Jabbari, M., Eftekhari, A., Farzipour, H. and Yousefian Jazi, S., 2023. Viral hemorrhagic septicemia virus (VHSV), past, present and future: a review. International Aquatic Research. 15(3): 191-203. doi: 10.22034/IAR.2023.1983457.1424 [ DOI] 6. Momeni, H., Fadaeifard, F., Momtaz, H. and Momeni, M., 2016. Nucleotide sequencing and phylogenetic analysis of glycoprotein gene of viral hemorrhagic septicemia virus (VHSV) isolated from rainbow trout in Chaharmahal and Bakhtiary province. Journal of Microbial Biology. 5(19): 1-10. doi: 10.22108/bjm.2016.21006 (In Persian) [ DOI] 7. Jonstrup, S.P., Kahns, S., Skall, H.F., Boutrup, T.S. and Olesen, N.J., 2013. Development and validation of a novel T aqman‐based real‐time RT‐PCR assay suitable for demonstrating freedom from viral haemorrhagic septicaemia virus. Journal of Fish Diseases. 36(1): 9-23. doi: 10.1111/j.1365-2761.2012.01416.x [ DOI] 8. Gharaguozlo, M., Ghajari, A., Abdi, K., Seifouri, P., Fallah Mehrabadi, M. and Shahbazian, N., 2018. Study on clinical and gross pathology manifestation of Viral Hemorrhagic Septicemia (VHS) disease in cultured rainbow trout (Oncorhynchus mykiss) in Iran (2013-2015). Iranian Scientific Fisheries Journal. 26(5): 121-129. doi: 10.2209/ISFJ.2017.115320 (In Persian) [ DOI] 9. Arian, A. and Torabi, M., 2016. Epidemiology of VHS disease in Iran and Khorasan Razavi Province, Fourth Iranian Ichthyology Conference. Mashhad. https://civilica.com/doc/1019607 (In Persian)
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Hadi Daneshi M. Identification and diagnosis of viral hemorrhagic septicemia (VHS) in Rainbow trout (Oncorhynchus mykiss) farms in Tehran province. , Journalaer. 2026; 2 (1) :61-66 URL: http://journalaer.com/article-1-86-en.html
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