Introduction:In Iran, Viral Hemorrhagic Septicemia (VHS) is one of the main causes of mortality of farmed trout in recent years. The use of theReal-Time PCR method will be significant in controlling and preventing rainbow trout viral diseases in the country. Therefore, this research investigated the identification and diagnosis of VHSV in rainbow trout farms in Tehran province. Materials & Methods:In this study, which was conducted in two phases, winter and spring 2022-3 and winter and spring 2023-4, by referring to rainbow trout breeding farms in Tehran province, samples were taken from fry weighing 0.3-3 was heated and using Quantitative Real-Time PCR method to identify and diagnose VHSV in rainbow trout farms in Tehran province. Results:According to the obtained results, VHS viral disease had the highest multiplication rate in the spring of 2024 in the fields and had a significant difference. In the results obtained in the winter of 2022 and the spring of 2023, the percentage of virus detection in breeding farms was the same, and these results were equal to the percentage of virus prevalence in the winter of 2024, and no significant difference was observed. According to the summary of the above results, the prevalence of the disease in Tehran province was reported as 9.8%. Conclusion:In the first three months of 2024, the disease had a clear and higher prevalence than last year, which is due to the increase of the disease in Takhir farms and needs more attention to improve biosecurity and disease control. VHS virus in circulation in the country depends on the weight, and often, the clinical form of disease and death does not occur in baby fish above 2 grams. Therefore, it seems that to establish an acceptable level of biosecurity system at the level of these farms, in addition to the necessary training for growers, more effective measures should be planned and implemented.
Hadi Daneshi M. Identification and diagnosis of viral hemorrhagic septicemia (VHS) in Rainbow trout (Oncorhynchus mykiss) farms in Tehran province. Journalaer 2026; 2 (1) :61-66 URL: http://journalaer.com/article-1-86-en.html