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The effect of processing lentil straw with urea, molasses and cellulase enzyme on chemical composition, gas production, ruminal and intestinal digestibility
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Zahra Abbasinavand   , Jamal Seifdavati * , Hossein Abdi benamar , Farzad Mirzaei Aghjeh Gheshlagh , Reza Seyedsharifi |
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Abstract: (768 Views) |
Introduction: The optimum use of unusual and non-conventional feed sources that do not compete with human food is necessary to provide animal feed. This research was conducted with the aim of investigating the effect of lentil straw processing with urea, molasses and enzyme on chemical composition, ruminal and intestinal digestibility and invitro gas production.
Materials & Methods: Lentil straws were processed with urea, molasses and enzymes. The enzyme source was fungal cellulase and rumen liquid. The samples chemical measurements, gas production and the digestibility was determined using the rumen and postruminal digestibility by the three-step method of Gargalo.
Results: In lentil straw, the addition of all three enzymes, molasses and the combination of different levels of molasses with rumen liquid and the mixture of two enzymes and the combination of all three with a level of 4% molasses increased the digestibility of dry matter in the entire digestive system. In lentil straw, addition of molasses, rumen liquid and combination of molasses with fungal cellulase enzyme and mixture of two enzymes with different levels of molasses increased rumen digestibility of crude protein (P<0.05). In lentil straw, adding molasses only in hours 2 and 4 after incubation, the combination of fungal cellulase and a mixture of two enzymes with all three levels of molasses in hours 2, 4 and 6 after incubation and in hours 8, 10, 48, 24, 12, 72 and 96 Hour after incubation with levels of 2 and 4% molasses, an increase in gas production was observed. The results showed that the addition of 2% molasses increased the gas production potential, while the enzyme decreased it. The addition of molasses and enzyme and the combination of molasses with a mixture of two enzymes increased the constant rate of decomposition. The results showed that molasses and enzyme increased the constant rate of decomposition and 4% molasses and enzyme increased the gas production potential. The combination of molasses with fungal cellulase and the mixture of two enzymes decreased the constant rate of decomposition and gas production potential. The average laboratory digestibility of organic matter, metabolizable energy, lactation energy and short chain fatty acids among all treatments in lentil straw was significant (P<0.05).
Conclusion: In general, biotransformation of lentil straw biomass with all three types of enzymes (fungal cellulase-ruminal liquid and combination of two enzymes) along with different levels of molasses could improve its nutritional value. |
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| Keywords: Cellulase enzyme, Digestibility, Lentil straw, Molasses, Rumen liquid, Urea |
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Full-Text [PDF 1198 kb]
(273 Downloads)
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Type of Study: Research |
Subject:
General
Received: 2025/09/24 | Accepted: 2025/09/24 | Published: 2025/12/2
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