Studying the effect of feeding Lactobacillus bulgaricus and Lactobacillus plantarum probiotics on the immune system and protection against the pathogen Aeromonas hydrophila in Zebrafish model

Ehsannia , Sheida; Ahari , Hamed; Kakolaki , Shapour; Anvar , Seyed Amir Ali; Yousefi , Seyedeh Shima

doi:10.22034/aer.1.2.77

[Home ] [Archive]

[ فارسی ]

Journal of Animal Environmental Research

Main Menu

Home

Journal Information

Articles archive

For Authors

For Reviewers

Registration

Contact us

Site Facilities

Search in website

Receive site information

Indexing

Volume 1, Issue 2 (8-2025)

Journalaer 2025, 1(2): 77-86

Back to browse issues page

Studying the effect of feeding Lactobacillus bulgaricus and Lactobacillus plantarum probiotics on the immune system and protection against the pathogen Aeromonas hydrophila in Zebrafish model

Sheida Ehsannia

, Hamed Ahari ^*

, Shapour Kakolaki

, Seyed Amir Ali Anvar

, Seyedeh Shima Yousefi

Abstract: (525 Views)

Introduction: Zebrafish is very similar to mammals in terms of physiology of digestive system, pharmacological and genetics. Also, the remarkable similarity of zebrafish immune system to mammals is as an alternative model for studying innate immunity and adaptive immunity as well as malignancies caused by immune cells. Therefore, this fish is an attractive model for investigating the basic inflammatory processes and damage to the intestine, which was used in this research and the results obtained can be generalized to humans. The purpose of this study is to investigate the effect of feeding with two probiotic strains of Lactobacillus bulgaricus and Lactobacillus plantarum separately and in combination on the immune system, the presence of bacteria in the intestine and protection against the pathogen Aeromonas hydrophila in the zebrafish model, which is a successful model for studies on vertebrate developmental biology, physical and biomedical research, intestinal bacterial colonization and immune responses have been introduced.
Materials & Methods: RT-PCR test was performed to prove the presence of probiotic bacteria in the intestine and quantitative evaluation of the expression of immune genes, and the mortality of fish after exposure to pathogenic bacteria was investigated. After preparation, probiotic bacteria were added to the feed at a concentration of 108 cfu/g at the rate of 3% of the body weight of the fish and were fed to 240 pieces of fish twice a day and the tests were evaluated for 60 (0, 28, 56 and 60) days.
Results: According to the results, the highest gene expression of Lactobacillus plantarum bacteria in the intestine in LA.pba treatment was on day 28, and the highest gene expression of Lactobacillus bulgaricus bacteria in LA.ba treatment was on day 60. The highest level of expression of immune genes IL1β and TNFα as inflammatory cytokines was in the normal group and even after exposure to the pathogen on day 60, the lowest level of expression of these genes was in the LA.pba group. Also, the highest survival rate was seen in the LA.pba group.
Conclusion: The obtained results indicate the appropriate effect of using two types of probiotics Lactobacillus plantarum and Lactobacillus bulgaricus in creating protection against the pathogen Aeromonas hydrophila. Also, it seems that the use of these two probiotics in combination has more beneficial effects in the zebrafish model.

Keywords: Zebra fish, Probiotic, Lactobacillus bulgaricus, Lactobacillus plantarum, Aeromonas hydrophila

Full-Text [PDF 826 kb] (132 Downloads)

Type of Study: Research | Subject: Special

References

1. 1. Agh, N., Irani, A., Noori, F. and Tookmehchi, A., 2019. Survey of Lactobacillus delbrueckii effects on Persian sturgeon in different life stages. Journal of Fisheries Science and Technology. 8(1): 1-8. https://doi. org/10.22113/jmst.2019.156745.22 27 (In Persian)

2. 2. Foo, H.L., Loh, T.C., Mutalib, N.E.A. and Rahim, R.A., 2019. The myth and therapeutic potentials of postbiotics. microbiome and metabolome in diagnosis, therapy and other strategic applications, 8th ed. Elsevier. 201-211.

3. 3. Wang, Y., Ren, Z., Fu, L. and Su, X., 2016. Two highly adhesive lactic acid bacteria strains are protective in zebrafish infected with Aeromonas hydrophila by evocation of gut mucosal immunity. Journal of Applied Microbiology. 120(2): 441-451. https://doi.org/10.1111/ jam.13002

4. 4. Brugman, S., 2016. The zebrafish as a model to study intestinal inflammation. Develoment & comparative immunology. 46: 82-92. https://doi.org/10.1016/j.dci.20 16.02.020

5. 5. Saddhe, A.A., Banerjee, G., Jamdadeh, R.A. and Thete, K.D., 2013. Zebrafish the reliable vertebrate model organism. DCSI. 91: 172-128.

6. 6. Fadai Raini, M., Ahmadifar, A. and Inayat Gholampour, T., 2017. Study of changing of chemical muscle content during maturation in Zebrafish (Danio rerio Hamilton, 1822) fed with prebiotic inulin. Journal of Animal Environment. 9(3): 245-252. https://doi.org/20.1001.1. 27171388.1396.9.3.32.2 (In Persian)

7. 7. Ali Azizi, R. and Ghaeni, M., 2016. The effect on the color supplement Chlorella sp. Algae of Danio rerio. Journal of Animal Environment. 8(2): 241-248. https:// doi.org/20.1001.1.27171388.1395.8.2.32.3 (In Persian)

8. 8. Saeedi, Z., Mirvaqefi, A.R., Abed Alam Dost, A.R., Farahmand, H. and Safari, R., 2020. Investigating sexual maturation and gonad morphohistology in Danio rerio zebrafish offspring under the influence of Lactobacillus plantarum probiotic. Scientific Journal of Iranian Fisheries. 29(2): 103-114. https://sid.ir/paper/ 4331/fa (In Persian)

9. 9. Morshedi, V., Agh, N., Marmazi, J., Nouri, F. and Mohammadiyan, T., 2018. Effects of Single and Combined Supplementation of Lactobacillus plantarum with dietary xylooligosaccharide on growth performance, body composition and physiological responses of sobaity (Sparidentex hasta) fingerling. Journal of Marine Science and Technology. 17(2): 44-57. https://doi.org/10. 22113/jmst.2016.40995 (In persian)

10. 10. Lin, Sh., Mao, Sh., Guan, Y., Luo, L. and Pan, Y., 2012. Effect of dietary chitosan oligosaccharides and Bacillus coagulans on the growth, innate immunity and resistanse of koi. Aquaculture. 342-343: 36-41. https:// doi.org/10.1016/j.aquaculture.20 12.02.009

11. 11. Zhang, C.H.N., Zhang, J.L., Guan, W.Ch., Zhang, Z.F., Guan, S.H., Zeng, Q.H., Cheng, G.F. and Cui, W., 2017. Effects of Lactobacillus delbrueckii on immune response, disease resistance against Aeromonas hydrophila, antioxidant capability and growth performance of Cyprinus carpio Huanghe var. Fish & Shellfish Immunology. 68: 84-91. https://doi.org/10.1016/j.fsi.20 17.07.012

12. 12. Tapia, P.A., Diaz, R.P. and Leon, R.J., 2012. Use or the probiotic Shewanella putrefaciens Pdp11on the culture of Senegalenses sole (Solea senegalensis, Kaup 1858) and gilthead seabream (Sparaus aurata), Aquaculture International. 21: 1-15. https://doi.org/10. 1007/s10499-012-9509-5

13. 13. Sepehrfar, D., Hoseinifar, S.H. and Jafarnodeh, A., 2019. The effects of singular or combined administration of Pediococcus acidlactii and Rafinose as prebiotic on mucosal immune parameters and intestinal histomorphology of Common Carp (Carassius auratus). Journal of Physiology and Animal Development. 13(4): 69-79. http://aqudev.liau.ac.ir/article-1547-en.html (In persian)

14. 14. Zakariaei, H., Sudagar, M., Hoseini, S.S., Paknejad, H. and Baruah, K., 2022. The effect of using synbiotics containing button mushroom (Agaricus bisporus) extract in combination with two species of lactic acid bacteria on digestive enzyme activities, body composition, growth and intestinal microbial flora in zebrafish. Journal of Marine Science and Technology. 21(2): 63-75. https:// doi.org/10.22113/jmst.2020.23318 3.2375 (In Persian)

15. 15. Rameshgar, M., Qomi Marzdashti, M.R., Hashemi Karoi, M., Hosseinifard, M. and Tabaripour, S.R., 2021. The effect of Lactobacillus plantarum and Bruis isolated from the digestive system of black fish (Capoeta razii) and its effect on the growth and safety indicators of common carp (Cyprinus carpio) in comparison with Primalac probiotic. Animal Physiology and Development Scientific Research. 14(2): 63-76. (In Persian)

16. 16. NguyenVan, N., Satoru Onodab, T., Van Khanh, T., Duy Hai, P., Thanh Trung, N., Hoang, L. and Koshio, Sh., 2019. Evaluation of dietary heat-killed Lactobacillus plantarum strain L-137 supplementation on growth performance, immunity and stress resistance of nile tilapia (Oreochromis niloticus). Aquaculture. 498: 371-379. https://doi.org/10.1016/j.aquaculture.2018.08.081

17. 17. Li, S., Guo, L., Si, X., Dai, Z., Zhou, Z. and Wu, Z., 2022. Lactobacillus plantarum WCFS1 alleviates Aeromonas hydrophila NJ-1induced inflammation and muscle loss in zebrafish (Danio rerio). Aquaculture. 548(1). https://doi.org/10.1016/j.aquaculture.2021.737603

18. 18. Mohammadian, T., Alishahi, M., Tabandeh, M.R., Ghorbanpoor, M., Gharibi, D., Tollabi, D. and Rohanizade, S., 2016. Probiotic effects of Lactobacillus plantarum and L. delbrueckii ssp. bulguricus on some immune-related parameters in Barbus grypus. Aquacult International. 24: 225-242. https://doi.org/10.1007/s104 99-015-9921-8

19. 19. Nobakht, F., Mohabatkar, H. and Behbahani, M., 2022. An Experimental Study of the Effects of Lactobacillus Acidophilus and Lactobacillus Plantarum Probiotic Bacteria on the Immune System, Growth Factors, and Disease Resistance in the Rainbow Trout. Biological Journal of Microorganism. 10(40): 115-126

20. 20. Ringo, E., Van Doan, H., Lee, S.H., Soltani, M., Hoseinifar, S.H., Harikrishnan, R. and Song, S.K., 2020. Probiotics, lactic acid bacteria and bacilli: interesting supplementation for aquaculture. Journal of Applied Microbiology. 129(1): 116-136. https://doi.org/ 10.1111/jam.14628

21. 21. Ehsannia, Sh., Ahari, H., Kakoolaki, Sh., Anvar, S.A. and Yousefi, Sh., 2023. Effects of probiotics on Zebrafish model infected with Aeromonas hydrophila: spatial distribution, antimicrobial, and histopathological investigation. BMC Microbiology. 22(1): 167. https:// doi.org/10.1186/s12866-022-02491-4

22. 22. Picchietti, S., Fausto, A.M., Randelli, E., Carnevali, O., Taddei, A.R. and Buonocore, F., 2009. Early treatment with Lactobacillus delbrueckii strain induces an increase in intestinal T-cells and granulocytes and modulates immune-related genes of larval Dicentrarchus labrax (L.) Fish Shellfish Immunol. 26: 368-376.

23. 23. Qin, C., Xie, Y., Wang, Y., Li, S., Ran, C., He, S. and Zhou, Z., 2018. Impact of Lactobacillus casei BL23 on the host transcriptome, growth and disease resistance in larval zebrafish. Frontiers in Physiology. 9: 1245. https://doi.org/10.3389/fphys.2018.01245

24. 24. Liu, W., Ren, P., He, S., Xu, L., Yang, Y., Gu, Z. and Zhou, Z., 2013. Comparison of adhesive gut bacteria composition, immunity, and disease resistance in juvenile hybrid tilapia fed two different Lactobacillus strains. Fish & Shellfish Immunology. 35(1): 54-62. https://doi.org/10.1016/j.fsi.2013.04.010

25. 25. Zivkovic, M., Hidalgo-Cantabrana, C., Kojic, M., Gueimonde, M., Golic, N. and Ruas-Madiedo, P., 2017. Capability of exopolysaccharide-producing Lactobacillus paraplantarum BGCG11 and its non producing isogenic strain NB1, to counteract the effect of enteropathogens upon the epithelial cell line HT29-MTX. Food Research International. 74: 199-207. https://doi.org/10.1016/j.foodres.2015.05.012

26. 26. Standen, B.T., Peggs, D.L., Rawling, M.D., Foey, A., Davies, S.J., Santos, G.A. and Merrifield, D.L., 2016. Dietary administration of a commercial mixed-species probiotic improves growth performance and modulates the intestinal immunity of tilapia, Oreochromis niloticus. Fish & Shellfish Immunology. 49: 427-435. https://doi. org/10.1016/j.fsi.2015.11.037

27. 27. Villamil, L., Reyes, C. and Martínez Silva, M.A., 2014. In vivo and in vitro assessment of Lactobacillus acidophilus as probiotic for tilapia (Oreochromis niloticus, Perciformes: Cichlidae) culture improvement. Aquaculture Research. 45(7): 1116-1125. https://doi.org/ 10.1111/are.12051

Send email to the article author

Add your comments about this article

‎ 10.22034/aer.1.2.77

Mendeley

Zotero

RefWorks

Ehsannia S, Ahari H, Kakolaki S, Anvar S A A, Yousefi S S. Studying the effect of feeding Lactobacillus bulgaricus and Lactobacillus plantarum probiotics on the immune system and protection against the pathogen Aeromonas hydrophila in Zebrafish model. , Journalaer. 2025; 1 (2) :77-86
URL: http://journalaer.com/article-1-146-en.html

Rights and permissions
	This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Volume 1, Issue 2 (8-2025)

Back to browse issues page

Persian site map - English site map - Created in 0.17 seconds with 39 queries by YEKTAWEB 4779