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:: Volume 1, Issue 4 (12-2025) ::
Journalaer 2025, 1(4): 59-68 Back to browse issues page
The Effect of Manganese on digestive enzymes and intestinal histology in Reared Young Beluga (Huso huso)
Fatemeh Hemmati , Hossein Khara * , Habib Vahaabzadeh Roudsari , Rezvanollah Kazemi
Abstract:   (1049 Views)
Introduction: This research aims to determine the effect of different amounts of dietary manganese on digestive enzymes and intestinal tissue in rearing young beluga (Huso huso).
Materials & Methods: For this purpose, 180 pieces of beluga were selected (respectively, with an average weight of 266±3.05 grams and a total length of 38.23±0.49 cm). In this study, six treatments and each treatment with three repetitions, with concentrations of 5 (Mn1), 10 (Mn2), 15 (Mn3), 20 (Mn4) and 25 (Mn5) mg of manganese sulfate monohydrate (MnSO4H2O) in each kilogram of diet and control (Mn0) without adding MnSO4 supplement was carried out at Dr. Beheshti's Sturgeon Breeding and Regeneration Center, Sanger Dam, Rasht city, for two months. After weighing, the desired amount of supplement was added to the factory powdered food along with deionized water. The fish were biometrically measured at the end of each month. Physical and chemical factors of water (temperature, oxygen and pH) were measured and recorded daily. At the end of the experiment, three pieces of fish were selected from each repetition and blood was taken from the caudal vein to measure digestive enzymes. Also, samples were taken from different parts of the intestine (anterior, middle and posterior) for histological studies.
Results: The results of the indices of digestive enzymes showed that the amount of amylase, lipase, and protease enzymes had a significant difference between some of the experimental treatments and the control group (p<0.05) and the maximum and minimum of these enzymes were observed in Mn2 and Mn5 treatments, respectively. In tissue studies, symptoms such as bleeding, hyperemia, necrosis, vacuolation were observed in different parts of the intestinal tissue in all treatments, even the control treatment, but the best intestinal tissue condition was in the Mn3 treatment.

Conclusion: According to the results of this study, the short-term addition of 10-15 mg of manganese per kilogram of diet of bluga improves the activity of digestive enzymes and also reduces intestinal tissue damage.
Keywords: Beluga (Huso huso), Dietry, Manganese sulfate, Digestive enzymes, Intestinal histology
Full-Text [PDF 1130 kb]   (340 Downloads)    
Type of Study: Research | Subject: General
References
1. 1. Asaikkutti, A., Bhavan, P.S., Vimala, K., Karthik, M. and Cheruparambath, P., 2016. Dietary supplementation of green synthesized manganese-oxide nanoparticles and its effect on growth performance, muscle composition and digestive enzyme activities of the giant freshwater prawn Macrobrachium rosenbergii. Journal of Trace Elements in Medicine and Biology. 35: 7-17. https://doi.org/10.1016/j.jtemb.2016.01.005
2. 2. Aschner, J.L. and Aschner, M., 2005. Nutritional aspects of manganese homeostasis. Molecular Aspects of Medicine. 26(4): 353-362.
3. 3. Au, D.W.T., 2004. The application of histo cytopathological biomarkers in marine pollution monitoring: a review. Marine pollution bulletin. 48(9-10): 817-834.‌ ttps://doi.org/10.1016/j.marpolbul.2004.02.032
4. 4. Barišić, J., Marijić, V.F., Mijošek, T., Čož-Rakovac, R., Dragun, Z., Krasnići, N. and Erk, M., 2018. Evaluation of architectural and histopathological biomarkers in the intestine of brown trout Salmo trutta Linnaeus, 1758 challenged with environmental pollution. Science of the total environment. 642: 656-664.‌ https://doi.org/10.1016/j.scitotenv.2018.06.045
5. 5. Bernfeld, P., 1955. Enzymes Carbohydrate Metabolism. In Methods in Enzymology Academic Press. 17: 149-158.
6. 6. Bonsignore, M., Manta, D.S., Mirto, S., Quinci, E.M., Ape, F., Montalto, V. and Sprovieri, M., 2018. Bioaccumulation of heavy metals in fish, crustaceans, molluscs and echinoderms from the Tuscany coast. Ecotoxicology and environmental safety. 162: 554-562.‌ https://doi.org/10.1016/j.ecoenv.2018.07.044
7. 7. Debnath, D., Pal, A.K., Sahu, N.P., Yengkokpam, S., Baruah, K., Choudhury, D. and Venkateshwarlu, G., 2007. Digestive enzymes and metabolic profile of Labeo rohita fingerlings fed diets with different crude protein levels. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 146(1): 107-114.‌ https://doi.org/10.1016/j.cbpb.2006.09.008.
8. 8. Farag, A.M., Nimick, D.A., Kimball, B.A., Church, S.E., Harper, D.D. and Brumbaugh, W.G., 2007. Concentrations of metals in water, sediment, biofilm, benthic macroinvertebrates, and fish in the Boulder River watershed, Montana, and the role of colloids in metal uptake. Archives of environmental contamination and toxicology. 52: 397-409.‌ https://doi.org/10.1007/s00244-0 05-0021-z
9. 9. Gómez-Requeni, P., Bedolla-Cázares, F., Montecchia, C., Zorrilla, J., Villian, M., Toledo-Cuevas, E.M. and Canosa, F., 2013. Effects of increasing the dietary lipid levels on the growth performance, body composition and digestive enzyme activities of the teleost pejerrey Odontesthes bonariensis. Aquaculture. 416: 15-22.‌ https://doi.org/10.1016/j.aquaculture.2013.08.027
10. 10. Halver, J.E., 2001. My 50 years in fish nutrition, 1949-99. Aquaculture Research. 32(8): 615-622.‌
11. 11. Hamlin, H.J., 2006. Nitrate toxicity in Siberian sturgeon Acipenser baeri. Aquaculture. 253(1): 688-693.‌ https://doi.org/10.1016/j.aquaculture.2005.08.025.
12. 12. Jain, K.L., 2009. Chronic effects of heavy metals on the activity of some digestive and metabolic enzymes in Cyprinus carpio. Annals of Biology. 25(1): 63-67.
13. 13. Kharkan, J., Sayadi, M.H., Hajiani, M., Rezaei, M.R., and Savabieasfahani, M., 2023. Toxicity of nickel oxide nanoparticles in Capoeta fusca, using bioaccumulation, depuration, and histopathological changes. Global Journal of Environmental Science and Management. 9(3): 427-444.‌ https://doi.org/10.22034/gjesm.2023.03.05 (In Pershian)
14. 14. Lall, S.P. and Milley, J.E., 2008. Trace mineral requirements of fish and crustaceans. Trace elements in animal production systems. 203-214.‌ https://doi.org/10. 3920/978-90-8686-638-0
15. 15. Lan, C.C. and Pan, B.S., 1993. In-vitro digestibility simulating the proteolysis of feed protein in the midgut gland of grass shrimp Penaeus monodon. Aquaculture. 109(1): 59-70.‌ https://doi.org/10.1016/0044-8486(93)904 86-I
16. 16. Liu, H., Fu, S., Zhang, S., Ding M. and Wang A., 2020. Lead induces structural damage, microbiota dysbiosis and cell apoptosis in the intestine of juvenile bighead carp Hypophthalmichthys nobilis. Aquaculture. 528: 735573. https://doi.org/10.1016/j.aquaculture.2020.735573
17. 17. Liu, Y., Wang, J.Y., Li, B.S., Qiao, H.J., Liu, X.D., Hao, T.T. and Wang, X.Y., 2018. Dietary manganese requirement of juvenile hybrid grouper, Epinephelus lanceolatus× E. fuscoguttatus. Aquaculture Nutrition. 24(1): 215-223. https://doi.org/10.1111/anu.12549
18. 18. Mansouri, B., Rahmani, R., Azadi, N.A., Davari, B., Johari, S.A. and Sobhani, P., 2015. Effect of waterborne copper oxide nanoparticles and copper ions on guppy Poecilia reticulata. Bioaccumulation and histopathology. Journal of Advances in Environmental Health Research. 3: 215 - 223. https://doi.org/10.22102/jaehr.2015.40205 (In Pershian)
19. 19. Mohseni, M., Pourali, H.R., Kazemi, R. and Bai, S.C., 2014. Evaluation of the optimum dietary protein level for the maximum growth of juvenile beluga Huso huso L. 1758. Aquaculture Research. 45(11): 1832-1841.‌ https://doi.org/10.1111/are.12134 (In Pershian)
20. 20. Muralisankar, T., Bhavan, P.S., Radhakrishnan, S., Seenivasan, C., Manickam, N. and Srinivasan, V., 2014. Dietary supplementation of zinc nanoparticles and its influence on biology, physiology and immune responses of the freshwater prawn, Macrobrachium rosenbergii. Biological trace element research. 160(1): 56-66.‌ https://doi.org/10.1007/s12011-014-0026-4
21. 21. Musharraf, M. and Khan, M.A., 2021. Dietary manganese requirement of fingerling Indian major carp, Labeo rohita (Hamilton) estimated by growth, tissue manganese concentration and hepatic manganese-superoxide dismutase activity. Aquaculture. 540: 734-736. https://doi.org/10.1016/j.aquaculture.2021.7367 34.
22. 22. Naz, S., Hussain, R., Ullah, Q., Chatha, A.M.M., Shaheen, A. and Khan, R.U., 2021. Toxic effect of some heavy metals on hematology and histopathology of major carp Catla catla. Environmental science and pollution research. 28: 6533-6539.‌ https://doi.org/10.1007/s11356. 020-10980-0.
23. 23. NRC (National Research Council). 2011. Nutrient Requirements of Fish and Shrimp. National Academies Press, Washington, DC, 376 + XVI.
24. 24. Ross, N.W., Firth, K.J., Wang, A., Burka, J.F. and Johnson, S.C., 2000. Changes in hydrolytic enzyme activities of naive Atlantic salmon Salmo salar skin mucus due to infection with the salmon louse Lepeophtheirus salmonis and cortisol implantation. Diseases of Aquatic Organisms. 41(1): 43-51.‌ https://doi: 10.3354/dao041043.
25. 25. Sadeghi, P., Tootooni, M.M. and Molaei, S., 2019. Use of Intestinal Tissue of Pomadasys kaakan and Lutjanus johnii as Biomarkers of Contamination in The Oman Sea.‌ (In Pershian)
26. 26. Sayadi, M.H. and Kharkan, J., 2022. Investigating the histological damage of different heavy metals on aqueduct fish Capoeta fusca. Aquaculture Sciences. 10(2): 149161.‌ (In Pershian)
27. 27. Sotoudeh, E., Amiri Behrosi, A., Bahadori, R., Habibi, H. and Morammazi, S., 2019. Nonspecific immune responses, activity of digestive enzymes and body composition of rainbow trout Oncorhynchus mykiss fed diets containing manganese sulfate nanoparticles. Journal of Animal Research Iranian Journal of Biology. 32(4): 269-282.‌ (In Pershian)
28. 28. Sharifpour, I., Hallajian, A. and Kazemi, R., 2014. Histology Laboratorial Techniques for Aquatics. Firs edition, Iranian Fisheries Research Organization, Tehran. 345 p. (In Pershian)
29. 29. Shaw, B.J., Al-Bairuty, G. and Handy, R.D., 2012. Effects of waterborne copper nanoparticles and copper sulphate on rainbow trout, Oncorhynchus mykiss: physiology and accumulation. Aquatic Toxicology. 116: 90-101.‌ https://doi.org/10.1016/j.aquatox.2012. 02.032
30. 30. Shihabi, Z.K. and Bishop, C., 1971. Simplified turbidimetric assay for lipase activity. Clinical chemistry. 17(12): 1150-1153.‌ https://doi.org/10.1093/clinchem/17. 12.1150.
31. 31. Tchounwou, P.B., Yedjou, C.G., Patlolla, A.K. and Sutton, D.J., 2012. Heavy metal toxicity and the environment. Molecular, clinical and environmental toxicology. Environmental toxicology. 3: 133-164.‌ https://doi.org/10.1007/978-3-7643-8340-4-6
32. 32. Torfi Mozanzadeh, M., Yaghoubi, M., Yavari, V., Agh, N., Marammazi, J.G. and Topic Popovic, N., 2015. Reference intervals for haematological and plasma biochemical parameters in sobaity sea bream juveniles Sparidentex hasta, Valenciennes 1830. Comparative Clinical Pathology. 24: 1501-1507.‌ https://doi.org/ 10.1007/s00580-015-2107-y (In Pershian)
33. 33. Verkleji J.A.S., 1993. The effects of heavy metals stress on higher plants and their use as biomonitors. Plant as bioindicators: indicators of heavy metals in the terrestrial environment. VCH, New York. 415- 424.
34. 34. Xiong, D.M., Xie, C.X., Zhang, H.J. and Liu, H.P., 2011. Digestive enzymes along digestive tract of a carnivorous fish Glyptosternum maculatum Sisoridae, Siluriformes. Journal of Animal Physiology and Animal Nutrition. 95(1): 56-64.‌ https://doi.org/10.1111/j.1439. 0396.2009.00984.x
35. 35. Yan, T., Teo, L.H. and Sin, Y.M., 1996. Effects of metals on {alpha}-amylase activity in the digestive gland of the green mussel, Perna viridis L. Bulletin of environmental contamination and toxicology. 56(4): 677-682.
36. 36. Ye, C.X., Tian, L.X., Yang, H.J., Liang, J.J., Niu, J. and Liu, Y.J., 2009. Growth performance and tissue mineral content of juvenile grouper Epinephelus coioides fed diets supplemented with various levels of manganese. Aquaculture Nutrition. 15(6): 608-614. https://doi.org/10. 1111/j.1365-2095.2008.00628.x
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Hemmati F, Khara H, Vahaabzadeh Roudsari H, Kazemi R. The Effect of Manganese on digestive enzymes and intestinal histology in Reared Young Beluga (Huso huso). , Journalaer. 2025; 1 (4) :59-68
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Volume 1, Issue 4 (12-2025) Back to browse issues page
پژوهش های محیط زیست جانوری Journal of Animal Environmental Research
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