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Effects of Yarrow (Achillea millefolium) and Pennyroyal (Mentha pulegium) Essential Oils on Blood Biochemical Parameters and Antioxidant Capacity in Commercial Laying Hens
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Hamed Jafarzadeh *   , Mohammadreza Sheikhlou , Rashid Safari |
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Abstract: (603 Views) |
Introduction: The essential oils of yarrow (Achillea millefolium) and pennyroyal (Mentha pulegium) contain bioactive compounds such as monoterpenes and sesquiterpenes, which exhibit antioxidant, anti-inflammatory, and metabolic regulatory properties. Given the restrictions on using antibiotic growth promoters in poultry, this study aimed to investigate the effects of different levels of yarrow and pennyroyal essential oils on serum biochemical parameters including total protein, albumin, total antioxidant capacity (TAC), malondialdehyde (MDA), glucose, and uric acid in commercial laying hens.
Materials & Methods: In this completely randomized design experiment, 128 Hyline W-80 laying hens were divided into 8 treatment groups: 1) Basal diet (negative control) 2) Basal diet + 0.125 mL emulsifier (positive control), 3) Basal diet supplemented with 50 mg/kg yarrow essential oil, 4) Basal diet supplemented with 100 mg/kg yarrow essential oil, 5) Basal diet supplemented with 150 mg/kg yarrow essential oil, 6) Basal diet supplemented with 50 mg/kg pennyroyal essential oil, 7) Basal diet supplemented with 100 mg/kg pennyroyal essential oil, 8) Basal diet supplemented with 150 mg/kg pennyroyal essential oil. After 6 weeks of feeding, 8 blood samples were collected from each group and biochemical parameters were measured using commercial kits. Data were analyzed using SPSS software with ANOVA.
Results: The highest levels of total protein and albumin were observed in the 150 mg/kg yarrow and 150 mg/kg pennyroyal groups (P>0.05). The maximum TAC was recorded in the 100 mg/kg pennyroyal group, indicating enhanced antioxidant defense. The lowest MDA and uric acid levels were found in the control and positive control groups (P>0.05). Blood glucose showed an insignificant increase in most essential oil-treated groups (P>0.05).
Conclusion: Findings suggest that yarrow and pennyroyal essential oils at 100 and150 mg/kg can serve as natural alternatives to antibiotics in layer diets by improving biochemical parameters and reducing oxidative stress. These compounds may enhance protein metabolism and antioxidant defense, potentially improving the quality of organic poultry products. Future studies should focus on elucidating the molecular mechanisms underlying these effects. |
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| Keywords: Achillea millefolium, Biochemical indices, Essential oil, Layers, Mentha pulegium |
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Type of Study: Research |
Subject:
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1. Bain, M.M., Nys, Y. and Dunn, I.C., 2016. Increasing persistency in lay and stabilising egg quality in longer laying cycles. What are the challenges? British Poultry Science. 57(3): 330-338. doi: 10.1080/00071668.2016.1161729 [ DOI] 2. Zeng, Z., Zhang, S., Wang, H. and Li, X., 2015. Essential oil and aromatic plants as feed additives in non ruminant nutrition: a review. Journal of Animal Science and Biotechnology. 6: 7. doi: 10.1186/s40104-015-0004-5 [ DOI] 3. Hashemipour, H., Kermanshahi, H., Golian, A. and Veldkamp, T., 2016. Effect of essential oils on performance, antioxidant status, and immune response in broiler chickens. Poultry Science. 95(10): 2178-2186. doi: 10.3382/ps/pew333 [ DOI] 4. Alagawany, M., El-Hack, M.E.A., Farag, M.R., Tiwari, R., Dhama, K. and Iqbal, H.M.N., 2021. The role of essential oils in poultry nutrition: Applications and prospects. Animals. 11(3): 500. doi: 10.3390/ani11030500 [ DOI] 5. Giannenas, I., Bonos, E., Christaki, E. and Florou Paneri, P., 2020. Phytogenic feed additives in poultry: Achievements and prospective applications. Animals. 10(5): 800. doi: 10.3390/ani10050800 [ DOI] 6. He, X., Hao, D., Liu, C., Zhang, X. and Xu, D., 2017. Effect of Supplemental Oregano Essential Oils in Diets on Production Performance and Relatively Intestinal Parameters of Laying Hens. American Journal of Molecular Biology. 7: 73-85. https://doi.org/10.4236/ajmb.2017.71006 [ DOI] 7. Abedini, A., Hassanabadi, A., Afzali, N. and Kermanshahi, H. 2017. The effect of different dietary levels of Pennyroyal (Mentha pulegium L.), probiotic and antibiotic on performance, carcass characteristics, and selected nutrients digestibility in broiler chickens. Archives of Medical Laboratory Sciences. 3(2): 15-22. https://doi.org/10.22037/amls.v3i2.17572 [ DOI] 8. Abdel-Wareth, A.A.A., Lohakare, J. and Südekum, K.H., 2019. Effects of thyme and oregano essential oils on performance, egg quality, and serum biochemistry in laying hens. Journal of Animal Physiology and Animal Nutrition. 103(2): 495-504. doi: 10.1111/jpn.13049 [ DOI] 9. El-Hack, M.E.A., Alagawany, M., Farag, M.R., Tiwari, R., Dhama, K. and Iqbal, H.M.N., 2020. Essential oils in poultry nutrition: An updated review. Poultry Science. 99(1): 1-10. doi: 10.3382/ps/pez456 [ DOI] 10. Gharibi, D., Ghasemi, H.A., Hajkhodadadi, I. and Nazaran, M.H., 2020. Antioxidant and hepatoprotective effects of yarrow essential oil in broiler chickens. Veterinary Medicine and Science. 6(4): 1-9. doi: 10.1002/vms3.345 [ DOI] 11. Boukhris, M., Bouaziz, M., Feki, I., Jemai, H. and Sayadi, S., 2019. Hypoglycemic and antioxidant effects of pennyroyal (Mentha pulegium) essential oil in diabetic rats. Journal of Medicinal Food. 22(3): 1-8. doi: 10.1089/jmf.2018.0123 [ DOI] 12. Rasouli, B., Movahhedkhah, S., Seidavi, A., Paz, E., Laudadio, V., Ayasan, T. and Mail, V.T., 2021. Effect of pennyroyal (Mentha pulegium L.) extract on performance, blood constitutes, immunity parameters, and intestinal microflora in broiler chickens. Indian Journal of Animal Science. 90(12): 1638-1643. https://doi.org/10.56093/ijans.v90i12.113202 [ DOI] 13. Gholamzadeh, S., Karimi, A. and Sadeghi, G.H., 2018. Antimicrobial effects of pennyroyal (Mentha pulegium) essential oil on poultry pathogens. Journal of Food Science and Technology. 55(6): 2345-2352. doi: 10.1007/s13197-018-3154-6 [ DOI] 14. Nielsen, B.L., Thomsen, M.G. and Sørensen, P., 2005. Blood plasma protein levels in chickens and their relation to performance. Poultry Science. 84(5): 789-795. doi: 10.1093/ps/84.5.789 [ DOI] 15. Ma, H., Zhang, H., Lv, Q., Zhao, X., Zhao, C. and Wang, Z., 2017. Chemical composition and antioxidant activities of essential oils of ginger (Zingiber officinale Roscoe). Journal of Food Measurement and Characterization. 11(4): 1285-1292. doi: 10.1007/s11694-017-9674-7 [ DOI] 16. Omidbaigi, R., Sefidkon, F. and Kazemi, F., 2007. The impact of yarrow (Achillea millefolium) on gastrointestinal health and performance of laying hens. Asian-Australasian Journal of Animal Sciences. 20(8): 1266-1272. doi: 10.5713/ajas.2007.1266 [ DOI] 17. Hossain, M.F., Islam, M.S. and Rahman, M.M., 2020. Plant extracts as a natural feed additive in poultry diets. Journal of Animal Physiology and Animal Nutrition. 104(5): 1369-1380. doi: 10.1111/jpn.13345 [ DOI] 18. Sharma, A., Kumar, S. and Tyagi, P.K., 2018. Physiological and biochemical changes in laying hens during heat stress. Animal Nutrition. 4(3): 265-272. doi: 10.1016/j.aninu.2018.03.003 [ DOI] 19. Khan, R.U., Naz, S. and Nikousefat, Z., 2013. Nutritional requirements of layer chickens. Journal of Animal Science and Technology. 55(3): 123-130. doi: 10.5187/jast.2013.55.3.123 [ DOI] 20. Ayaz, F.A., Glew, R.H., Huang, H.S., Chuang, L.T. and VanderJagt, D.J., 2019. Phytochemical compositions and antioxidant activities of different plant extracts. Journal of Food Science. 84(5): 1234-1241. doi: 10.1111/1750-3841.14612 [ DOI] 21. Akhter, M.Z., Islam, M.S. and Rahman, M.M., 2012. Effects of herbal extracts on serum biochemical parameters in laying hens. Asian-Australasian Journal of Animal Sciences. 25(12): 1733-1740. doi: 10.5713/ajas.2012.12345 [ DOI] 22. Alavi, S.M., Gheisari, A. and Toghyani, M., 2019. Influence of thyme essential oil on serum biochemistry and performance in layers. Poultry Science. 98(12): 6785-6792. doi: 10.3382/ps/pez456 [ DOI] 23. Mahboubi, M. and Haghi, G., 2008. Antimicrobial activity and chemical composition of Mentha pulegium L. essential oil. Journal of Ethnopharmacology. 119(2): 325-327. doi: 10.1016/j.jep.2008.07.023 [ DOI] 24. Brenes, A. and Roura, E., 2010. Essential oils in poultry nutrition: Main effects and modes of action. Animal Feed Science and Technology. 158(1-2): 1-14. doi: 10.1016/j.anifeedsci.2010.03.007 [ DOI] 25. Tadić, V., Arsić, I., Zvezdanović, J., Zugić, A., Cvetković, D. and Pavkov, S., 2017. Phytochemical profile and biological activities of Achillea millefolium essential oil. Journal of Ethnopharmacology. 206: 1-10. doi: 10.1016/j.jep.2017.05.002 [ DOI] 26. Sahin, K., Orhan, C., Smith, W. and Kucuk, O., 2018. The role of dietary phytogenic additives in poultry nutrition. Journal of Animal Physiology and Animal Nutrition. 102(1): 1-12. doi: 10.1111/jpn.12800 [ DOI] 27. Boehm, G., Kien, C.L. and Morowitz, M.J., 2018. Influence of dietary essential oils on gut microbiota in poultry. Poultry Science. 97(3): 925-933. doi: 10.3382/ps/pex372 [ DOI] |
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Jafarzadeh H, Sheikhlou M, Safari R. Effects of Yarrow (Achillea millefolium) and Pennyroyal (Mentha pulegium) Essential Oils on Blood Biochemical Parameters and Antioxidant Capacity in Commercial Laying Hens. , Journalaer. 2026; 2 (3) :35-42 URL: http://journalaer.com/article-1-140-en.html
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