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:: Volume 1, Issue 1 (4-2025) ::
Journalaer 2025, 1(1): 33-40 Back to browse issues page
Antibacterial peptides have attracted a- great deal of attention due to their biological properties against a wide range of microorganisms
Maryam Shokoohmand Dr. * , Hossein Zolgharnein Dr. , Mohammad Ali Salari Ali Abadi Dr. , Mojtaba Alishahi Dr. , Reza Safari Dr.
Abstract:   (191 Views)
Introduction: The present study aimed to investigate antibacterial and properties of proteins produced by the enzyme hydrolysis of lantern fish (Benthosema petrotum) produced by alcalase and flavourzyme enzymes.
Materials & Methods: Membrane ultrafiltration method was used to isolate and purify antimicrobial peptides smaller than 3 kDa. Antibacterial activity of protein hydrolyzed with alkalase enzyme (BPHA) and flavourzyme enzyme (BPHF) against Aeromonas hydrophila, Streptococcus agalactiae, Sterptococcus iniae, Yersinia ruckeri (standard strains) was performed by disk diffusion and microdilution method.
Results: The results showed the appropriate efficiency of enzymes used to isolate antimicrobial peptides smaller than 3 kDa from lantern fish. Also, the lowest amount of MIC, MBC of hydrolyzed protein by flavourzyme against bacteria S, iniae was 16 and 32 μg / ml. The diameter of the growth inhibition zone of peptides isolated with alkalase enzyme (BPHA) and flavourzyme enzyme (BPHF) against Y. ruckeri and S. iniae was competitive with tetracycline antibiotic.
Conclusion: In general, it can be concluded that bioactive peptides derived from lantern fish have relative antibacterial effects against fish bacterial agents, although this effect depends on the type of enzyme used and the bacterial species. Therefore, further research on the possibility of using these antibacterial agents for fish bacterial pathogens is recommended.
Keywords: Aeromunas hydrophyila, Antibacterial properties, Bioactive peptides, Lantern fish, Streptococcus agalactiae, Sterptococcu iniae, Yersini ruckeri
Full-Text [PDF 741 kb]   (60 Downloads)    
Type of Study: Research | Subject: Special
References
1. 1. Gao, R., Yu, Q., Shen, Y., Chu, Q., Ge, C., Fen, S., Yang, M., Yuan, L., McClements, D.J. and Sun, Q., 2021. Production, bioactive properties, and potential applications of fish protein hydrolysates: Developments and challenges. Trends Food Sci Technol. 110: 687-699. doi: https://doi.org/10.1016/j.tifs.2021.02.031
2. 2. Adel, M., Pourgholam, R., Zoriehzahra, S.J., Safari, R. and Ghiasi, M., 2015. The effect of different level of Mentha piperita on growth performance, body composition, Intestinal bacteria and survival rate of Oncorhynchus mykiss in challenging with Yersinia ruckeri. Aquat Ecol. 4(4): 70-62.
3. 3. Mortazi, M., Omidzahir, S. and Akhoundian, M., 2021. Study the occurrence of Aeromonas hydrophila septicemia in Carassius gibelio of Shazde River of Babolsar. Veterinary Researches & Biological Products. 34(1): 140-147. doi: 10.22092/VJ.2020.128309.1645
4. 4. Zorriehzahra, M.J., Adel, M. and Torabi Delshad, S., 2017. Enteric redmouth disease: Past, present and future: A review. Iran J Fish Sci. 17(4): 1135-1156. doi: 10.22092/IJFS.2018.114726
5. 5. Sepahdari, A., Saeedi, A.S., Kakoulaki, S., Habibi Kotanaee, F. and Babaalian, A.R., 2014. Incidence of streptococcusis in rainbow trout (Onchorhynchus mykiss) farms in Haraz River in Mazandaran Province, Iran. Iran J Fish Sci. 22(4): 41-50. doi: 20.1001.1.10261354.1392. 22.4.4.9
6. 6. Zorriehzahra, M.J., Mehrabi, M. and Rezvani, S., 2014. Introducing of the most important rearing Tilapia infectious diseases (bacterial and viral) in hatchery phase till offer to the market (Grow out phase). J Ornam Aquat. 1(3): 9-20. doi: 20.1001.1.24234575.1393.1.3.2.1
7. 7. Harikrishnan, R., Nisha, M.R. and Balasundaram, C., 2003. Hematological and biochemical parameters in common carp, Cyprinus carpio, followingherbal treatment for Aeromonas hydrophila infection. Aquaculture. 221
8. (1-4): 41-50. doi: https://doi.org/10.1016/S0044-8486(03) 00023-1
9. 8. Ucak, I., Afreen, M., Montesano, D., Carrillo, C., Tomasevic, I., Simal-Gandara, J. and Barba, F.J., 2021. Functional and bioactive properties of peptides derived from marine side streams. Mar Drugs. 19(2): 71. doi: https://doi.org/10.3390/md19020071
10. 9. Rabiei, S., Nikoo, M., Rezaei, M. and Rafieian-Kopaei, M., 2018. A review on therapeutic effects of marine bioactive peptides in animal models and human. Iranian Journal of Physiology and Pharmacolog. 2(4): 213-201.
11. 10. Javaherdoust, S., Yeganeh, S. and Keramat Amirkolaie, A., 2019. Effects of dietary rainbow trout (Oncorhynchus mykiss) viscera protein hydrolysate on some hematological and blood serum biochemical parameters of rainbow trout juvenile. Iran Fish Sci J. 28(2): 71-83. doi: 10.22092/ISFJ.2019.118925
12. 11. Guerard, F., Guimas, L. and Binet, A., 2002. Production of tuna waste hydrolysates by a commercial neutral protease preparation. J Mol Catal B Enzym. 19: 489-498. doi: https://doi.org/10.1016/S1381-1177(02)00 203-5
13. 12. Bi, J., Tian, C., Jiang, J., Zhang, G.L., Hao, H. and Hou, H.M., 2020. Antibacterial activity and potential application in food packaging of peptides derived from turbot viscera hydrolysate. J Agric Food Chem. 68(37): 9968-9977. doi: https://pubs.acs.org/doi/10.1021/acs.jafc. 0c03146.
14. 13. Anil, K.T. and Sunil, K., 2016. Biochemical characterisation and antibacterial properties of fish skin mucus of freshwater fish (Hypophthalmichthys nobilis). Int J Pharm Pharm Sci. 8(6): 6-10.
15. 14. Robert, M., Zatylny-Gaudin, C., Fournier, V., Corre, E., Le Corguillé, G., Bernay, B. and Henry, J., 2015. Molecular characterisation of peptide fractions of a Tilapia (Oreochromis niloticus) byproduct hydrolysate and in vitro evaluation of antibacterial activity. Process Biochem. 50(3): 487-92. doi: https://doi.org/10.1016/j. procbio.2014.12.022
16. 15. Sila, A., Nedjar-Arroume, N., Hedhili, K., Chataigné, G., Balti, R., Nasri, M., Dhulster, P. and Bougatef, A., 2014. Antibacterial peptides from barbel muscle protein hydrolysates: Activity against some pathogenic bacteria. LWT-Food Sci Technol. 55(1): 183-188. doi: https://doi.org/10.1016/j.lwt.2013.07.021
17. 16. Valinassab, T., Pierce, J. and Johannesson, K., 2006. Lantern fish (Benthosema pterotum) resources as a target for commercial exploitation in the Oman Sea. J Appl Ichthyol. 23(5): 573-577. doi: https://doi.org/10.1111/j. 1439-0426.2007.01034.x
18. 17. Zahuranec, B., Karuppasamy, P.K., Valinassab, T., Kidwai, S., Bernardi, J. and Bernardi, G., 2012. Cryptic speciation in the mesopelagic environment: Molecular phylogenetics of the lanternfish genus Benthosema. Mar Genom. 7: 7-10. doi: https://doi.org/ 10.1016/j.margen.2012.05.001
19. 18. Chai, H.J., Chan, Y.L., Li, T.L., Chen, Y.C., Wu, C.H., Shiau, C.Y. and Wu, C.J., 2012. Composition characterization of Myctophids (Benthosema pterotum): Antioxidation and safety evaluations for Myctophids protein hydrolysates. Int Food Res J. 46(1): 118-126. doi: https://doi.org/10.1016/j.foodres.2011.12.008
20. 19. Shaviklo, A. and Moradi, Y., 2019. Supplying nutritional needs of livestock and humans from lanternfishes. Iran Fish Sci J. 28(4): 90-96. doi: 10.22092/ ISFJ.2019.119466
21. 20. Asgarzadeh, F., Ataei, M. and Choobkar, N., 2020. Comparative comparison of physicochemical properties of gelatin extracted from lantern fish (Benthosema pterotum, Alcock 1890) and Bovine Gelatin. Iran Fish Sci J. 29(2): 53-63. doi: 10.22092/ISFJ.2020.120956
22. 21. Association of Official Analytical Chemists (AOAC). 2002. Official Methods of Analysis Chemists 14th ed. Washington DC.
23. 22. Ojagh, S.M., Abdollahzadeh, E., Shabanpour, B., Kordjazi, M. and Khosravi, G.R., 2017. Effectiveness of bioactive compounds produced by Lactobacillus lactis in combination with essential oils, salts and acetic acid to control Listeria monocytogenes in liquid model and minced meat of hypophthalmichthys molitrix. Aquatic Physiology and Biotechnology. 4(4): 89-110. doi: 20. 1001.1.23453966.1395.4.4.7.0
24. 23. Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J., 1951. Protein measurement with the Folin phenol reagent. J Biol Chem. 193(1): 265-275.
25. 24. Raghavan, S. and Kristinsson, H.G., 2008. Antioxidative efficacy of alkali-treated tilapia protein hydrolysates: A comparative study of five enzymes. J Agric Food Chem. 56(4): 1434-1441. doi: https://doi.org/ 10.1021/jf0733160
26. 25. Azizi, R., Motallebi, A., Nazemi, M., Sharifruhani, M. and Afsharnasab, M., 2020. Survey of antibacterial properties of extracts of green algae ulva fasciata collected from Persian Gulf. J Anim Environ. 12(3):
27. 469-474. doi: 10.22034/AEJ.2020.121633 (In Persian)
28. 26. Ghotaslou, R., Saghati, H., Dehnad, A. and Salahi Eshlaghi, B., 2016. Antibacterial effects of Azerbaijan honey on Pseudomonas aeruginosa biofilm. Iran J Med Microbiol. 9(4): 40-46.
29. 27. Siddik, M.A., Howieson, J., Fotedar, R. and Partridge, G.J., 2021. Enzymatic fish protein hydrolysatesin finfish aquaculture: a review. Rev Aquac. 13(1): 406-430. doi: https://doi.org/10.1111/raq.12481
30. 28. Montesano, D., Gallo, M., Blasi, F. and Cossignani, L., 2020. Biopeptides from vegetable proteins: New scientific evidences. Curr Opin Food Sci. 31: 31-37. doi: https://doi.org/10.1016/j.cofs.2019.10.008
31. 29. Ovissipour, M., Rasco, B., Shiroodi, S.G., Modanlow, M., Gholami, S. and Nemati, M., 2013. Antioxidant activity of protein hydrolysates from whole anchovy sprat (Clupeonella engrauliformis) prepared using endogenous enzymes and commercial proteases. J Sci Food Agric. 93(7): 1718-1726. doi: https://doi.org/10.1002/jsfa.5957
32. 30. Henriques, A., Vázquez, J.A., Valcarcel, J., gério Mendes, R.O., Bandarra, M.N. and Pires, C., 2021. Characterization of protein hydrolysates from fish discards and by-products from the North-West Spain fishing fleet as potential sources of bioactive peptides. Mar Drugs. 19(6): 338. doi: https://doi.org/10.3390/ md19060338
33. 31. Ovissipour, M., Abedian, A., Motamedzadegan, A., Rasco, B., Safari, R. and Shahiri, H., 2009. The effect of enzymatic hydrolysis time and temperature on the properties of protein hydrolysates from Persian sturgeon (Accipenser persicus) viscera. Food Chem. 115(1):
34. 238-242. doi; https://doi.org/10.1016/j.foodchem.2008.12. 013
35. 32. Yasemi, M., Ghomi Marzdashti, M.R., Darnahal, T., Mohammadzadeh, B. and Amini, H., 2013. Yelid of protein recovery and degree of hydrolysis associated protein hydrolysates from Bighead Carp (Aristichthys nobilis) by using enzymes. Iran Fish Sci J. 22(1):
36. 149-156. doi: http://hdl.handle.net/1834/36758
37. 33. Cerrato, A., Capriotti, A.L., Capuano, F., Cavaliere, C., Montone, A.M.I., Montone, C.M., Piovesana, S., Zenezini Chiozzi, R. and Laganà, A., 2020. Identification and antimicrobial activity of medium-sized and short peptides from yellowfin tuna (Thunnus albacares) simulated gastrointestinal digestion. Foods. 9(9): 1185. doi: https://doi.org/10.3390/foods9091185
38. 34. Payam, B., Soltani, M., Shamsaie Mehrgan, M., Rajabi Islami, H. and Nazemi, M., 2021. Antibacterial activity of sea cucumber (Holothuria leucospilota) extracts on Lactococcus garvieae and Aeromonas hydrophila. J Anim Environ. 13(3): 307-314. doi: 10.22034/AEJ.2020.254363.2387 (In Persian)
39. 35. Salavati Khoshghalb, M., Moshfegh, A. and Setorki, M., 2020. Evaluation of Antibacterial Characteristics of Hemolymph of Cerastoderma and Didacna Bivalves in the southern Coasts of Caspian Sea. J Anim Environ. 12(1): 369-374. doi: 10.22034/AEJ.2020.105614 (In Persian)
40. 36. Baco, N., Oslan, S.N.H., Shapawi, R., Mohhtar, R.A.M., Noordin, W.N.M. and Huda, N., 2022. Antibacterial activity of functional bioactive peptides derived from fish protein hydrolysate. IOP Conf Ser Earth Environ Sci. 967(1): 012019. doi: https://doi.org/10. 1088/1755-1315/967/1/012019
41. 37. Marshall, S.H. and Arenas, G., 2003. Antimicrobial peptides: A natural alternative to chemical antibiotics andapotential for applied biotechnology. Electron J Biotechnol. 6(3): 96-109.
42. 38. Bagley, C.P., 2014. Potential role of synthetic antimicrobial peptides in animal health to combat growing concerns of antibiotic resistance, a review. Wyno Academic Journal of Agricultural Sciences. 2(2): 19-28.
43. 39. Cotter, P.D., Ross, R.P. and Hill, C., 2013. Bacteriocins -a viable alternative to antibiotics? Nat Rev Microbiol. 11(2): 95-105. doi: https://doi.org/10.1038/nrmicro2937
44. 40. Pezeshk, S., Ojagh, S.M., Rezaei, M. and Shabanpour, B., 2019. Fractionation of protein hydrolysates of fish waste using membrane ultrafiltration: investigation of antibacterial and antioxidant activities. Probiotics Antimicrob Proteins. 11(3): 1015-1022. doi: https://doi.org/10.1007/s 12602-018-9483-y
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Shokoohmand M, Zolgharnein H, Salari Ali Abadi M A, Alishahi M, Safari R. Antibacterial peptides have attracted a- great deal of attention due to their biological properties against a wide range of microorganisms. Journalaer 2025; 1 (1) :33-40
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Volume 1, Issue 1 (4-2025) Back to browse issues page
پژوهش های محیط زیست جانوری Journal of Animal Environmental Research
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